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Optimization of ultraviolet irradiation of Clarias gariepinus (Burchell, 1822) eggs for androgen production

Published online by Cambridge University Press:  15 January 2021

Victor Tosin Okomoda Open the ORCID record for Victor Tosin Okomoda [Opens in a new window] ,
Haziqah Jumahat Nursyaza ,
Ijabo Oga Samuel ,
Anuar Hassan ,
Abraham Sunday Oladimeji ,
Ambok Bolong Abol-Munafi ,
Korede Isaiah Alabi  and
Mhd Ikhwanuddin
Victor Tosin Okomoda*
Affiliation:
Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Haziqah Jumahat Nursyaza
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Ijabo Oga Samuel
Affiliation:
Department of Aquaculture, Faculty of Agriculture, Federal University of Gashua, P.M.B 1005Ilorin, Nigeria
Anuar Hassan
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Abraham Sunday Oladimeji
Affiliation:
Agricultural Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria
Ambok Bolong Abol-Munafi
Affiliation:
Department of Aquaculture, Faculty of Agriculture, Federal University of Gashua, P.M.B 1005Ilorin, Nigeria Faculty of Food Science and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Korede Isaiah Alabi
Affiliation:
Department of Agricultural Extension and Management, Federal College of Forestry, JosPlateau, Nigeria
Mhd Ikhwanuddin*
Affiliation:
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
*
Authors for correspondence: Victor Tosin Okomoda. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria. Tel: +23 48033319959. E-mail: okomodavictor@yahoo.com Ikhwanuddin Mhd. Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Tel: +60 199851868. E-mail: ikhwanuddin@umt.edu.my
Authors for correspondence: Victor Tosin Okomoda. Department of Fisheries and Aquaculture, College of Forestry and Fisheries, University of Agriculture Makurdi, P.M.B. 2373Makurdi, Nigeria. Tel: +23 48033319959. E-mail: okomodavictor@yahoo.com Ikhwanuddin Mhd. Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Tel: +60 199851868. E-mail: ikhwanuddin@umt.edu.my
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Summary

The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C. gariepinus and cold shocked at 5°C for 5 min just moments before cell cleavage. Ploidy analysis was performed using karyotype chromosome counting. The results obtained suggested that the further the distance, the better the hatchability rate, however prolonged duration seemed to significantly reduced hatchability. All treatments with surviving progenies at the end of the study showed evidence of successfully diploid gynogen (2n = 56) induction at different percentages. However, the optimal protocol that gave a moderately high hatchability/survival rate and completely induced gynogens was exposure of the eggs to UV irradiation at 20 cm for 1 min. It was concluded that the distance and duration of UV irradiation affects gynogenetic induction in African catfish C. gariepinus.

Keywords

African catfishChromosome manipulationCold shockUV radiation
Type
Research Article
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 223 - 228
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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