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Genetic diversity in spring faba bean (Vicia faba L.) genotypes as revealed by high-throughput KASP SNP markers

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Abstract

Faba bean is one of the oldest legume crop grown around the world for its cheap source of protein and for being used in crop rotation with cereals to improve soil fertility through nitrogen fixation. This study was designed to determine the extent and pattern of genetic diversity and relationships among 48 Ethiopian faba bean genotypes using 37 SNPs loci based on Kompetitive allele specific PCR SNP markers. Thirty-six SNPs were found polymorphic and revealed an average of 95.6% polymorphisms. The gene diversity ranged from 0.16 to 0.50 with a mean of 0.42 and the PIC value ranged from 0.14 to 0.38 with a mean of 0.33. The Bayesian clustering model grouped the genotypes into two genetically distinct clusters with certain degree of admixture, indicating the introduction of chromosomes of different ancestry and allele frequency. The result clearly showed the presence of relatively high genetic diversity among faba bean genotypes grown in Ethiopia. Thus, these genotypes can be used in faba bean breeding programs to develop farmer preferred cultivars with desirable traits.

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All information regarding this work is presented in the write up of the manuscript, supplementary Table 1 and supplementary Table 2 at the end of this manuscript.

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Acknowledgements

This work is a part of the first author’s MSc thesis. The authors would like to thank Addis Ababa University, Oromia Agricultural Research Institute (OARI), Sinana Agricultural Research Center, Holeta Agricultural Research Center, and Kulumsa Agricultural Research Center for materials support.

Funding

This work was financially funded by Oromia Agricultural Research Institute (OARI) and Africa RISING Project (ILRI) through International Center for Agricultural Research in Dry Areas (ICARDA).

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Authors and Affiliations

  1. Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

    Behailu Mulugeta & Kassahun Tesfaye

  2. Sinana Agricultural Research Center, Oromia Agricultural Research Institute, P. O. Box 208, Bale-Robe, Ethiopia

    Behailu Mulugeta

  3. Ethiopian Biotechnology Institute, P.O. Box 5954, Addis Ababa, Ethiopia

    Kassahun Tesfaye

  4. Holeta Agricultural Research Center, Ethiopian Institute of Agricultural Research (EIAR), Holeta, Ethiopia

    Gemechu Keneni

  5. International Center for Agricultural Research in Dry Areas, INRA-Quich Rue Hafiane Cherckaoui, Agdal Rabat-Instituts, Station Exp, Rabat, Morocco

    Seid Ahmed

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  1. Behailu Mulugeta
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  2. Kassahun Tesfaye
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  3. Gemechu Keneni
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  4. Seid Ahmed
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All authors participated in conducting data mining, selecting KASP SNP primers, data analysis, designing the experiment, interpreting the data, drafting and revising the manuscript; and approved the final manuscript.

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Correspondence to Behailu Mulugeta.

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Mulugeta, B., Tesfaye, K., Keneni, G. et al. Genetic diversity in spring faba bean (Vicia faba L.) genotypes as revealed by high-throughput KASP SNP markers. Genet Resour Crop Evol 68, 1971–1986 (2021). https://doi.org/10.1007/s10722-021-01110-x

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