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Identification and Differential Expression of microRNA in Response to Elevated Phospholipase Cγ Expression in Liver RH 35 Carcinoma Cells

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Abstract

Our study has primarily shown the positive effect of PLCγ2 on liver tumor cell proliferation, but the molecular basis for its function remains elusive. miRNAs have been widely accepted as important modulators of various cellular activities. This study attempts to characterize the global influence of PLCγ2 on miRNA expressions in liver cancer RH35 cells. Firstly, the recombinant adenovirus AdPLCγ2 was infected into the cells. High-throughput sequencing technology was applied to measure miRNA expressions in PLCγ2-overexpressing cells. Moreover, the target genes and signaling pathways modulated by PLCγ2-specifc miRNAs were identified using target prediction program, GO annotation and KEGG analysis. As a result, totally 246 known and 1075 novel candidate miRNAs were identified, among which 34 known and 191 novel miRNAs exhibited ≥2-fold changes in the AdPLCγ2-infected cells. Correspondingly, 6985 target genes of above 225 differently-expressed miRNAs were predicted, mainly involved in Hippo signaling, Wnt signaling etc., and responsible for tumor development, cell proliferation, apoptosis, migration, lipid metabolism and so on. In aggregate, PLCγ2 induces the significant alterations in miRNA expression, thus providing mechanistic insights into tumorgenesis mediated by PLCγ2, and maybe offers some clues on identifying potential candidates for controlling liver cancer cell growth.

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Funding

This study was supported by the National Natural Science Foundation of China (no. 31401209), Ministry of Science and Technology of the People’s Republic of China (MOST) and Student Research Training Program (SRTP) of Henan University of Science and Technology (no. 2018368).

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  1. Animal Science and Technology School, Henan University of Science and Technology, 471023, Luoyang, China

    Xiaoguang Chen, Xuemin Zhu, Zhiguo Wei & Qiongxia Lv

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  1. Xiaoguang Chen
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  2. Xuemin Zhu
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Correspondence to Xiaoguang Chen.

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Chen, X., Zhu, X., Wei, Z. et al. Identification and Differential Expression of microRNA in Response to Elevated Phospholipase Cγ Expression in Liver RH 35 Carcinoma Cells. Cytol. Genet. 54, 555–565 (2020). https://doi.org/10.3103/S009545272006002X

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