Abstract
MiR-150-5p is an immune-related miRNA and elevated in the plasma of patients with aplastic anemia (AA), but its role in T cell activation in patients with severe aplastic anemia (SAA) is unclear. This study aims to explore the role of miR-150-5p in T cell activation of SAA. RT-PCR and Western blot were used to detect the expression of mRNA and protein. The cell proportion was detected by flow cytometry. The lentiviruses expressing miR-150-5p inhibitor and Bach2 shRNA were respectively infected to produce stable miR-150-5p or Bach2 knockout cells. Brdu incorporation method was used to detect T cell proliferation. SAA mouse model was induced with cyclophosphamide and busulfan, and intravenous injection of LV inhibitor NC and LV-miR-150-5p inhibitor. The miR-150-5p expression is up-regulated in SAA, which is negatively correlated with Bach2. Inhibition of miR-150-5p reduces the activation of T cells. MiR-150-5p directly targeted 3′UTR of Bach2. Moreover, the expression of miR-150-5p and the activation of T cells were decreased in SAA mouse model. MiR-150-5p promotes T cell activation in SAA by targeting Bach2. Targeting miR-150-5p may be a new molecular therapy for SAA patients.
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This study was supported by grants from National Natural Science Foundation of China (U1804192) and Key Scientific Research Project Plan of Institutions of Higher Learning of Henan Province (19A320046).
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Li, Y., Yu, J., Wang, F. et al. MiR-150-5p regulate T cell activation in severe aplastic anemia by targeting Bach2. Cell Tissue Res 384, 423–434 (2021). https://doi.org/10.1007/s00441-020-03373-9
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DOI: https://doi.org/10.1007/s00441-020-03373-9