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Peroxidase Mimicking Activity of Palladium Nanocluster Altered by Heparin

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Abstract

NADH, composed of metal binding, charged and redox groups, acts as a “three-in-one” template to construct peroxidase mimicking Pd nanoclusters (NCs). The physicochemical properties of Pd NCs are highly dependent upon the molar ratio of [NADH]/[Na2PdCl4], thereby contributing to distinct peroxidase mimicking activities. The ultrasmall Pd nanozyme that contains 30% metallic Pd0 species, exhibits the Km of 0.063 mM toward TMB and 80.8 mM toward H2O2. Notably, addition of heparin enables the enhancement of its peroxidase-like activity in neutral media. A colorimetric assay was well-established at pH 6 for quantitatively monitoring heparin in aqueous solution and biological fluid. The linear response lies in the range of 0.5–25 µg mL−1, with the limit of detection of 1.1 ng mL−1. This work paves a promising pathway to manufacture highly active enzyme mimetics with desirable physicochemical properties.

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Acknowledgements

This study was funded by National Natural Science Foundation of China (21878225, 21776215).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Jinnan District, 135 Yaguan Road, Tianjin, 300350, P. R. China

    Qianqian Zhang, Shanshan Zheng, Jinli Zhang, Wei Li & Yan Fu

  2. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, P. R. China

    Qianqian Zhang, Shanshan Zheng, Jinli Zhang, Wei Li & Yan Fu

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  1. Qianqian Zhang
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Correspondence to Yan Fu.

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Zhang, Q., Zheng, S., Zhang, J. et al. Peroxidase Mimicking Activity of Palladium Nanocluster Altered by Heparin. Catal Lett 151, 2537–2546 (2021). https://doi.org/10.1007/s10562-021-03530-x

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