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Momordica cymbalaria improves reproductive parameters in alloxan-induced male diabetic rats

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Abstract

Male reproductive dysfunction is one of the common complications of diabetes mellitus that causes infertility. This study was designed to investigate the protective effect of Momordica cymbalaria (M. cymbalaria) extracts on diabetes mediated reproductive toxicity in male Wistar rats. The induction of diabetes was performed using a single intraperitoneal injection of alloxan (120 mg/kg). Skin and seed extracts (250 and 500 mg/kg) of M. cymbalaria were orally administered to alloxan-induced diabetic male rats for 28 days. Postprandial blood glucose (PBG) levels were recorded at 7-day interval for four weeks. The effects of the treatment on blood glucose, weight of reproductive organs, sperm count, testosterone levels, antioxidant capacity, and histomorphology were evaluated. Treatment with the above extracts of M. cymbalaria significantly (p < 0.05) improved the reproductive parameters as well as the antioxidant levels superoxide dismutase (SOD) and glutathione-s-transferase (GST) in the diabetic rats. Also, oral treatment with M. cymbalaria extracts significantly reduced the PBG and malondialdehyde (MDA) levels. Further, it revived the histomorphology of reproductive organs in diabetic rats. Interestingly, skin extract at a dose of 500 mg/kg was found to be more efficient in elevating the level of testosterone and sperm count in the diabetic rats. Based on the results, it is clear that M. cymbalaria not only regulates the postprandial blood glucose levels but also improves the reproductive health in the diabetic state.

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Acknowledgments

The authors are thankful to UGC-SAP, MHRD-RUSA and DST-FIST for providing the instrumental facility to perform this research work.

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Authors and Affiliations

  1. Department of Environmental Biotechnology, Bharathidasan University, Tamil Nadu, Tiruchirappalli, 620024, India

    Abbirami Elangovan, Siva Durairaj, Abinaya Subramanian, Sooraj Ramakrishnan, Dinesh Kumar Lakshmanan, Guna Ravichandran & Sivasudha Thilagar

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  1. Abbirami Elangovan
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  2. Siva Durairaj
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  3. Abinaya Subramanian
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  4. Sooraj Ramakrishnan
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  5. Dinesh Kumar Lakshmanan
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  7. Sivasudha Thilagar
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Contributions

EA performed the animal studies, interpreted the data and wrote the manuscript. DS undertook the histological examination of the reproductive organs. SA participated in the statistical analysis. RS and EA have conducted all in vivo experiments. LDK and RG carried out extract preparations as well as assisted in the animal studies. TS designed and supervised the work. All authors read and approved and the manuscript.

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Correspondence to Sivasudha Thilagar.

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The experimental design was approved by Institutional Animal Ethical Committee (Registration No.779/PO/Re/S/03/ CPCSEA) and the study was performed according to the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA).

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Elangovan, A., Durairaj, S., Subramanian, A. et al. Momordica cymbalaria improves reproductive parameters in alloxan-induced male diabetic rats. 3 Biotech 11, 76 (2021). https://doi.org/10.1007/s13205-020-02612-8

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