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40 years of glyco-polyacrylamide in glycobiology

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Abstract

Polyacrylamide conjugates of glycans have long been widely used in many research areas of glycobiology, mainly for immobilizing glycans in solid-phase assays and as multivalent inhibitors. Pending biotin tag allows immobilizing Glyc-PAA quantitatively on any surface, and acts as a tracer for detection of carbohydrate-binding proteins. However, the scope of already realized capabilities of these probes is immeasurably richer than those listed above. This review is not so much about routine as about less common, but not less significant applications. Also, the data on the glycopolymers themselves, their molecular weight, size and polymer chain flexibility are presented, as well as the methods of synthesis, clusterisation and entropy factor in their interaction with proteins.

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Abbreviations

6’SLN:

Neu5Acα2-6Galβ1-4GlcNAc

biot:

Biotin residue

BSA:

Bovine serum albumin

DOPE:

Dioleoylphosphatidyl ethanolamine

ELISA:

Enzyme-linked immunosorbent assay

Fluo:

Fluorescein residue

Glyc:

Carbohydrate residue, usually spacer (sp) armed

HRP:

Horse-radish peroxidase

mol%:

Molar percent

NHS:

N-hydroxysuccinimide

PAA:

Polyacrylamide

PGA:

Printed glycan array

TF:

Thomsen-Friedenreich antigen

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Acknowledgments

The work was supported by the Russian Science Foundation grant № 20-63-47110.

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., Moscow, 117997, Russia

    Alexander Tuzikov, Alexander Chinarev, Nadezhda Shilova, Elena Gordeeva, Oxana Galanina, Tatyana Ovchinnikova & Nicolai Bovin

  2. Centre for Kode Technology Innovation, Auckland University of Technology, Auckland, 1010, New Zealand

    Marcel Schaefer & Nicolai Bovin

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  1. Alexander Tuzikov
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Tuzikov, A., Chinarev, A., Shilova, N. et al. 40 years of glyco-polyacrylamide in glycobiology. Glycoconj J 38, 89–100 (2021). https://doi.org/10.1007/s10719-020-09965-5

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