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Application of extracellular polymers on soil communities exposed to oil and nickel contamination

  • Environmental Microbiology - Research Paper
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Abstract

The petrochemical industry is responsible for many accidental releases of pollutants in soil such as hydrocarbons and toxic metals. This co-contamination is responsible for a delay in the degradation of the organic pollution. Many successful technologies to remove these metals apply extracellular polymeric substances (EPS). In this study, we tested the application of an EPS from a Paenibacillus sp. to aid the bioremediation of soils contaminated with crude oil and nickel. We conducted a microcosm experiment to soils containing combinations of oil, nickel, and EPS. The final concentration of oil was evaluated with an infrared spectrometer. Also, we sequenced the metagenomes of the samples in an ion torrent sequencer. The application of EPS did not aid the removal of hydrocarbons with or without the presence of nickel. However, it led to a smaller decrease in the diversity indexes. EPS decreased the abundance of Actinobacteria and increased that of Proteobacteria. The EPS also decreased the connectivity among Actinobacteria in the network analysis. The results indicated that the addition of EPS had a higher effect on the community structure than nickel. Altogether, our results indicate that this approach did not aid the bioremediation of hydrocarbons likely due to its effect in the community structure that affected hydrocarbonoclastic microorganisms.

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Authors and Affiliations

  1. Department of Pharmacy, University São Francisco, R. Waldemar César da Silveira, 105 - Jardim Cura D’ars, Campinas, SP, 13045-510, Brazil

    Natália Franco Taketani

  2. Coordination of Metallurgical and Environmental Process, Centre of Mineral Technology, Av. Pedro Calmon, 900 - Cidade Universitária, Rio de Janeiro, RJ, 21941-908, Brazil

    Natália Franco Taketani, Rodrigo Gouvêa Taketani, Andrea Camardella de Lima-Rizzo & Cláudia Duarte da Cunha

  3. Department of Soil Sciences, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil

    Rodrigo Gouvêa Taketani & Fernando Dini Andreote

  4. Biochemistry Department, Chemistry School, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Selma Gomes Ferreira Leite

  5. Environmental Microbiology, Embrapa Environment, Jaguariúna, SP, Brazil

    Itamar Soares Melo

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  1. Natália Franco Taketani
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  2. Rodrigo Gouvêa Taketani
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  7. Cláudia Duarte da Cunha
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Corresponding authors

Correspondence to Natália Franco Taketani, Rodrigo Gouvêa Taketani or Cláudia Duarte da Cunha.

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Taketani, N.F., Taketani, R.G., Leite, S.G.F. et al. Application of extracellular polymers on soil communities exposed to oil and nickel contamination. Braz J Microbiol 52, 651–661 (2021). https://doi.org/10.1007/s42770-021-00428-z

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  • DOI: https://doi.org/10.1007/s42770-021-00428-z

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