Abstract
Peroxisomes, being indispensable organelles, play an important role in different biological processes in eukaryotes. PEX33, a filamentous fungus-specific peroxin of the docking machinery of peroxisomes, is involved in the virulence and development of other fungal pathogens. However, it is not clear whether PEX33 is necessary for the pathogenicity and development of an insect pathogenic fungus. In the present study, we report the presence of homologs of PEX33, namely MrPEX33 (MAA_05331), in the entomopathogenic fungus, Metarhizium robertsii. An M. robertsii transgenic strain expressing the fusion protein with MrPEX33-GFP and mCherry-PTS1 showed that MrPEX33 localizes to peroxisomes. The results also demonstrated that MrPEX33 is involved in the peroxisomal import pathway by peroxisomal targeting signals. Targeted gene deletion of MrPEX33 led to a significant decline in the asexual sporulation capacity, which was accompanied by downregulation of several conidiation-associated genes, such as wetA, abaA, and brlA. More importantly, our bioassay results showed that the virulence of ∆MrPEX33 mutants, against Galleria mellonella through cuticle infection, was greatly reduced. This was further accompanied by a significant drop in appressorium formation and cuticle penetration. Additionally, ∆MrPEX33 mutants showed a significant decrease in tolerance to cell wall integrity and oxidative stress. Taken together, our results suggest that MrPEX33 is involved in the cuticle infection-related morphogenesis and pathogenicity.
Key points
• MrPEX33 is a specific peroxin of the docking machinery of peroxisomes.
• MrPEX33 localizes to peroxisomes and is involved in the import of matrix proteins.
• MrPEX33 is involved in the pathogenicity associated with cuticle infections.
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Data availability
All data generated or analyzed during this study are included in this published article (and its supplementary information files).
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Funding
The research was jointly supported by the National Natural Science Foundation of China (grant numbers: 31772226, 31572060, and 31972332), the Key Project for Natural Science Research of Anhui Provincial Higher School (Grant numbers: KJ2019A0192), the Project for Excellent Young Talents in Universities of Anhui Province (grant numbers: gxyqZD2020007), and the Innovation Foundation for Postgraduates of Anhui Agricultural University (grant numbers: 2020ysj-7).
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Conceived and designed the experiments: ZW and BH. Performed the experiments: JF, YJ, XX, and LX. Analyzed the data: XX, LX, and QZ. Contributed reagents/materials/analysis tools: JF, YJ, XX, LX, and QZ. Wrote the paper: ZW and BH. All authors read and approved the final manuscript.
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Wang, Z., Feng, J., Jiang, Y. et al. MrPEX33 is involved in infection-related morphogenesis and pathogenicity of Metarhizium robertsii. Appl Microbiol Biotechnol 105, 1079–1090 (2021). https://doi.org/10.1007/s00253-020-11071-3
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DOI: https://doi.org/10.1007/s00253-020-11071-3