Abstract
Cassava is an important starchy and food crop; however, the commercial value of cassava is seriously constrained by postharvest physiological deterioration (PPD). In this study, the molecular mechanism underlying PPD was studied by comparative physiological and transcriptomic analyses. Physiological analyses indicated that the contents of H2O2 and malondialdehyde (MDA), and the activities of superoxide dismutase (SOD) and catalase (CAT) all increased along with PPD development. Further transcriptomic analysis investigated the distribution of the differential expression profiling and identified a total of 10,347 differentially expressed genes (DEGs) during PPD. Notably, most of the genes encoding antioxidant enzymes and protein kinases showed significant upregulation at transcriptional levels, indicating the activation of antioxidant system and kinase-mediated signaling pathway during the PPD process. In addition, the genes involved in the starch synthesis pathway were repressed, whereas the genes associated with the starch degradation pathway were induced, thus leading to a decrease in starch content during the PPD process. Together, this study yields new insight into the transcriptional regulation network during the PPD process and provides potential gene resources for the delay of PPD in cassava.
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13 November 2021
A Correction to this paper has been published: https://doi.org/10.1007/s11105-021-01322-6
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Funding
This work was supported by the National Natural Science Foundation of Hainan Province (2019RC290), the National Natural Science Foundation of China (31801419), the 2020 Research Program of Sanya Yazhou Bay Science and Technology City (SKJC-2020-2-002), the Central Public-Interest Scientifc Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences (1630052017021, 1630012019009, 1630052019023), and the Central Public-Interest Scientific Institution Basal Research Fund for the Innovative Research Team Program of the Chinese Academy of Tropical Agricultural Sciences (17CXTD-28, 1630052017017), and the earmarked fund for the Modern Agro-industry Technology Research System (CARS-11-HNGJC).
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Data curation, Yan Yan and Sihan Zhao; formal analysis, Yan Yan; funding acquisition, Yan Yan; investigation, Sihan Zhao; software, Zehong Ding and Weiwei Tie; supervision, Wei Hu; writing—original draft, Yan Yan; writing—review & editing, Wei Hu. All authors have read and agreed to the published version of the manuscript.
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Key Messages
• The changes of physiological indexes during PPD process were analyzed.
• A total of 10347 differentially expressed genes were identified during PPD process.
• The DEGs involved in antioxidant system, starch metabolism, and protein kinases were identified during PPD.
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Yan, Y., Zhao, S., Ding, Z. et al. Comparative Transcriptomic Analysis of Storage Roots in Cassava During Postharvest Physiological Deterioration. Plant Mol Biol Rep 39, 607–616 (2021). https://doi.org/10.1007/s11105-020-01274-3
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DOI: https://doi.org/10.1007/s11105-020-01274-3