Abstract
Alkaline stress is one of the severe abiotic stresses, which is not well studied so far, especially among cyanobacteria. To affirm the characteristics of alkaline stress and the subsequent adaptive responses in Arthrospira platensis NIES-39 and Arthrospira platensis PCC 7345, photosynthetic pigments, spectral properties of thylakoids, PSII and PSI activities, and pigment-protein profiles of thylakoids under different pH regimes were examined. The accessory pigments showed a pH-mediated sensitivity. The pigment-protein complexes of thylakoids are also affected, resulting in the altered fluorescence emission profile. At pH 11, a possible shift of the PBsome antenna complex from PSII to PSI is observed. PSII reaction center is found to be more susceptible to alkaline stress in comparison to the PSI. In Arthrospira platensis NIES-39 at pH 11, a drop of 68% in the oxygen evolution with a significant increase of PSI activity by 114% is recorded within 24 h of pH treatment. Alterations in the cellular ultrastructure of Arthrospira platensis NIES-39 at pH 11 were observed, along with the increased number of plastoglobules attached with the thylakoid membranes. Arthrospira platensis NIES-39 is more adaptable to pH variation than Arthrospira platensis PCC 7345.
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Abbreviations
- CBB G-250:
-
Coomassie Brilliant Blue G-250
- Chl:
-
Chlorophyll
- DCMU:
-
1,1-Dichloro methyl diphenyl urea
- DCPIP:
-
2,5-Dichlorophenol indophenol
- LiDS:
-
Lithium dodecyl sulfate
- MV:
-
Methyl viologen
- NIES:
-
National Institute of Environmental Studies
- pBQ:
-
para-Benzoquinone
- PBsomes:
-
Phycobilisomes
- PC:
-
Phycocyanin
- PCC:
-
Pasteur Culture Collection
- PEC:
-
Phycoerythrocyanin
- PSI:
-
Photosystem I
- PSII:
-
Photosystem II
- TEM:
-
Transmission electron microscopy
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Monika M. Jangir acknowledges the financial support from the Department of Science & Technology, Government of India in the form of DST-INSPIRE fellowship. The authors wish to thank BITS, Pilani, for providing infrastructural facilities.
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Monika M. Jangir received financial support from the Department of Science & Technology, Government of India in the form of DST-INSPIRE fellowship from December 2013 to 2018.
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B. Vani conceived the original idea. B. Vani and Shibasish Chowdhury supervised the findings and experiments of this work. Monika M. Jangir performed the experimental works, literature surveys, and initial manuscript writing. All authors equally provided critical feedback and helped shape the research, analysis, and final manuscript.
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Jangir, M.M., Chowdhury, S. & Bhagavatula, V. Differential response of photosynthetic apparatus towards alkaline pH treatment in NIES-39 and PCC 7345 strains of Arthrospira platensis. Int Microbiol 24, 219–231 (2021). https://doi.org/10.1007/s10123-021-00160-6
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DOI: https://doi.org/10.1007/s10123-021-00160-6