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Development and Characterization of Amikacin Loaded Emulgel for the Treatment of Mycetoma

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Pharmaceutical Chemistry Journal Aims and scope

The objective of this work was to develop and characterise an amikacin sulfate loaded emulgel to be used as a co-adjuvant treatment for mycetoma caused by principal agents Actinomadura madurae and Nocardia brasiliensis. Development of the amikacin sulfate emulgel was conducted in 3 stages: (1) formulation of the emulsion containing amikacin; (2) selection of the proportion of pluronic F-127 necessary to form a gel; and (3) formation of the emulgel. The emulgel was characterised by the values of pH, relative density, accelerated stability, viscosity, extensibility, adhesiveness, cumulative amount of amikacin, and skin permeation (kp and flux). The proportion of emulsifiers in the emulsion was 10% and 0.77% (w/w) of amikacin, and the emulsion was stable when subjected to the centrifugal test. Agel with 30% pluronic F-127 and an average adhesiveness of 2.7 mJ was used to form the emulgel. The formulated amikacin sulfate emulgel has the following properties: pH 5.5; relative density, 0.907; accelerated stability (centrifugation); extensibility. 923.72 mm2; adhesiveness, 1.27 mJ; cumulative amikacin (within 24 h), 34.13%; F = 105 μg/cm2/h; kp = 0.016 cm/h; and tLag = 14.75 h. The emulgel formulation contains 0.77% of amikacin and demonstrates appropriate technical characteristics to be used as a complement to systemic administration for the treatment of mycetoma.

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Acknowledgments

This work was supported by UNAM-PAPIIT IG 100220. Dr. Escobar Chávez wants to acknowledge PAPIME PE 201420 and Cátedra PIAPI 2015.

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Authors and Affiliations

  1. Facultad de Estudios Superiores Cuautitlán-Universidad Nacional Autónoma de México, Unidad de Investigación Multidisicplinaria, Laboratorio 12: Sistemas transdérmicos, Carretera Cuautitlán Teoloyucan, km 2.5 San Sebastián Xhala, C. P 54714, Cuautitlán Izcalli, Estado de México, México

    Karla Stella Constantino-González, Pablo Serrano-Castañeda, Omar Rodrigo Guadarrama-Escobar, Miriam López-Cervantes, Clara Luisa Domínguez-Delgado & José Juan Escobar-Chávez

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  1. Karla Stella Constantino-González
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  2. Pablo Serrano-Castañeda
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  3. Omar Rodrigo Guadarrama-Escobar
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  4. Miriam López-Cervantes
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  5. Clara Luisa Domínguez-Delgado
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  6. José Juan Escobar-Chávez
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Correspondence to José Juan Escobar-Chávez.

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Constantino-González, K.S., Serrano-Castañeda, P., Guadarrama-Escobar, O.R. et al. Development and Characterization of Amikacin Loaded Emulgel for the Treatment of Mycetoma. Pharm Chem J 54, 1024–1032 (2021). https://doi.org/10.1007/s11094-021-02315-1

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  • DOI: https://doi.org/10.1007/s11094-021-02315-1

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