Abstract
Vegetable by-products represent a valuable source of compounds with biological activity that could be used in food and cosmetics industries. The objective of this work is the valorization through the lipophilic nutraceuticals characterization and in vitro antioxidant activity of Jalapeño pepper industrial by-product oil, and the remainder flour from oil extraction. Jalapeño pepper by-products and whole fruit from two regions: Chihuahua and Sinaloa States from Mexico were obtained from a local industry. From the whole fruit it was prepared a simulated raw by-product, in order to use it to evaluate the effect of industrial scalding process on the by-product. The Jalapeño pepper by-product contain around 22% of oil with important lipophilic nutraceuticals, the oil has a greenness color, and remains attractive concentrations of essential fatty acids, more than 70% of linoleic acid; β-carotene up to 1.85 mg/100 g of oil; α-tocopherol (46.12 mg/100 g of oil) up to four times more than red pepper seeds (C. annuum). Moreover, two and a half and five times more capsaicin and dihydrocapsaicin, respectively, were detected in the oil fraction, than the reported before in no polar extract from whole fruit of C. frutescens. The scalding process reduces the β-carotene, capsaicinoids, and total phenolic content and antioxidant activity of the oil fraction, but despite of this, Jalapeño pepper by-product oil and remainder flour contain bioactive compounds at similar concentrations to previously reports in other peppers. The Jalapeño pepper by-products derived by the food industry have the potential to be used for the recovery of an oil with nutraceutical potential.
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Orellana-Escobedo, L., Garcia-Amezquita, L.E., Olivas, G.I., Cervantes-Paz, J.J., Sepulveda, D.R.: Capsaicinoids content and proximate composition of Mexican chili peppers (Capsicum spp.) cultivated in the State of Chihuahua. CyTA J. Food. (2013). https://doi.org/10.1080/19476337.2012.716082
SIAP. Servicio de Información Agroalimentaria y Pesquera.: Atlas Agroalimentario. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. http://infosiap.siap.gob.mx:8080/agricola_siap_gobmx/ResumenProducto.do (2019). Accessed 4 Jan 2020
Sandoval-Castro, C.J., Valdez-Morales, M., Perea-Domínguez, X.P., Medina-Godoy, S., Espinosa-Alonso, L.G.: Antioxidant activity of processed and raw seed byproduct from Jalapeño pepper. J. Chem. Biol. Phys. Sci. 4(5), 26–34 (2014)
Sandoval-Castro, C.J., Valdez-Morales, M., Oomah, B.D., Gutiérrez-Dorado, R., Medina-Godoy, S., Espinosa-Alonso, L.G.: Bioactive compounds and antioxidant activity in scalded Jalapeño pepper industrial byproduct (Capsicum annuum). J. Food Sci. Technol. (2017). https://doi.org/10.1007/s13197-017-2636-2
Chouaibi, M., Rezig, L., Hamdi, S., Ferrari, G.: Chemical characteristics and compositions of red pepper seed oils extracted by different methods. Ind. Crops Prod. (2019). https://doi.org/10.1016/j.indcrop.2018.11.030
Cervantes-Paz, B., Yahia, E.M., Ornelas-Paz, J.J., Victoria-Campos, C.I., Ibarra-Junquera, V., Pérez-Martínez, J.D., Escalante-Minakata, M.P.: Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeño peppers at intermediate stages of ripening. Food Chem. (2014). https://doi.org/10.1016/j.foodchem.2013.09.060
Moreno-Escamilla, J.O., de la Rosa, L.A., López-Díaz, J.A., Rodrigo-García, J., Núñez-Gastélum, J.A., Alvarez-Parilla, E.: Effect of the smoking process and firewood type in the phytochemical content and antioxidant capacity of red Jalapeño pepper during its transformation to chipotle pepper. Food Res. Int. (2015). https://doi.org/10.1016/j.foodres.2015.07.031
Baenas, N., Belovic, M., Ilic, N., Moreno, D.A., García-Viguera, C.: Industrial use of pepper (Capsicum annuum L.) derived products: technological benefits and biological advantages. Food Chem. (2019). https://doi.org/10.1016/j.foodchem.2018.09.047
Gurnani, N., Gupta, M., Mehta, D., Mehta, B.K.: Chemical composition, total phenolic and flavonoid contents, and in vitro antimicrobial and antioxidant activities of crude extracts from red chilli seeds (Capsicum frutescens L.). J. Taibah Univ. Sci. (2016). https://doi.org/10.1016/j.jtusci.2015.06.011
Ma, Y., Wu, X., Zhao, L., Wang, Y., Liao, X.: Comparison of the compounds and characteristics of pepper seed oil by pressure-assisted, ultrasound-assisted and conventional solvent extraction. Innov. Food Sci. Emerg. Technol. 54, 78–86 (2019). https://doi.org/10.1016/j.ifset.2019.03.011
Echave, J., Pereira, A.G., Carpena, M., Prieto, M.A., Simal-Gandara, J.: Capsicum seeds as a source of bioactive compounds: biological properties, extraction systems, and industrial application. In: Dekebo, A. (ed.) Capsicum. IntechOpen, London (2020)
Jahan, N., Rahman, K.: Cold pressed capia pepper (Capsicum annuum L.) seed oil. In: Ramadan, M.F. (ed.) Cold pressed oils: green technology, bioactive compounds, functionality, and applications, pp. 440–446. Academic Press, Elsevier, New York (2020)
Yilmaz, E., Hüriyet, Z., Arifoglu, N., Dilek Dündar, E.: Functional properties of the capia pepper defatted press cakes. Waste Biomass Valoriz. (2017). https://doi.org/10.1007/s12649-016-9602-5
Samia Azabou, S., Taheur, F.B., Jridi, M., Bouaziz, M., Nasri, M.: Discarded seeds from red pepper (Capsicum annum) processing industry as a sustainable source of high added-value compounds and edible oil. Environ. Sci. Pollut. Res. (2017). https://doi.org/10.1007/s11356-017-9857-9
Romo-Hualde, A., Yetano-Cunchillos, A.I., González-Ferrero, C., Sáiz-Abajo, M.J., González-Navarro, C.J.: Supercritical fluid extraction and microencapsulation of bioactive compounds from red pepper (Capsicum annum L.) by-products. Food Chem. (2012). https://doi.org/10.1016/j.foodchem.2012.01.062
El Ksibi, I., Ben, Slama R., Faidi, K., Ben, Ticha M., M´henni, M.K.: Mixture approach for optimizing the recovery of colored phenolics from red pepper (Capsicum annum L.) by-products as potential source of natural dye and assessment of its antimicrobial activity. Ind. Crops Prod. (2015). https://doi.org/10.1016/j.indcrop.2015.03.017
Tae-I, K.: Preparation method of natural condiments prepared by using pepper seed. Patent KR101844130B1 (2018). https://patents.google.com/patent/KR101844130B1/. Accessed 10 Oct 2020
Meghvansi, M.K., Siddiqui, S., Khan, M.H., Gupta, V.K., Vairale, M.G., Gogoi, H.K., Singh, L.: Naga chilli: a potential source of capsaicinoids with broad-spectrum ethnopharmacological applications. J. Ethnopharmacol. (2010). https://doi.org/10.1016/j.jep.2010.08.034
Fernández-Bedmar, Z., Alonso-Moraga, A.: In vivo and in vitro evaluation for nutraceutical purposes of capsaicin, capsanthin, lutein and four pepper varieties. Food Chem. Toxicol. (2016). https://doi.org/10.1016/j.fct.2016.10.011
Jarret, R.L., Levy, I.J., Potter, T.L., Cermak, S.C.: Seed oil and fatty acid composition in Capsicum spp. J. Food Compos. Anal. (2013). https://doi.org/10.1016/j.jfca.2013.02.005
Yilmaz, E., Sevgi Arsunar, E., Aydeniz, B., Güneser, O.: Cold pressed capia pepper seed (Capsicum annuum L.) oils: composition, aroma, and sensory properties. Eur. J. Lipid Sci. Technol. (2015). https://doi.org/10.1002/ejlt.201400276
Richard, D., Kefi, K., Barbe, U., Bausero, P., Visioli, F.: Polyunsaturated fatty acids as antioxidants. Pharmacol. Res. (2008). https://doi.org/10.1016/j.phrs.2008.05.002
Castillo, R.L., Zepeda, A.B., Short, S.E., Figueroa, E., Bustos-Obregón, E., Farías, J.G.: Protective effects of polyunsaturated fatty acids supplementation against testicular damage induced by intermittent hypobaric hypoxia in rats. J. Biomed. Sci. (2015). https://doi.org/10.1186/s12929-015-0112-8
Roohbakhsh, A., Karimi, G., Iranshahi, M.: Carotenoids in the treatment of diabetes mellitus and its complications: a mechanistic review. Biomed. Pharmacother. (2017). https://doi.org/10.1016/j.biopha.2017.04.057
Ornelas-Paz, J.J., Martínez-Burrola, J.M., Ruiz-Cruz, S., Santana-Rodríguez, V., Ibarra-Junquera, V., Olivas, G.I., Pérez-Martínez, J.D.: Effect of cooking on the capsaicinoids and phenolics contents of Mexican peppers. Food Chem. (2010). https://doi.org/10.1010/j.foodchem.2009.09.054
Álvarez-Parrilla, E., de la Rosa, L.A., Amarowicz, R., Shahidi, F.: Antioxidant activity of fresh and processed Jalapeño and Serrano peppers. J. Agric. Food Chem. (2011). https://doi.org/10.1021/jf103434u
Cervantes-Paz, B., Yahia, E.M., Ornelas-Paz, J.J., Gardea-Béjar, A.A., Ibarra-Junquera, V., Pérez-Martínez, J.D.: Effect of heat processing on the profile of pigments and antioxidant capacity of green and red jalapeño peppers. J. Agric. Food Chem. (2012). https://doi.org/10.1021/jf303091u
FAO. Food and Agriculture Organization: Global Initiative on Food Loss and Waste Reduction-SAVE FOOD. https://www.unece.org/fileadmin/DAM/trade/agr/meetings/wp.07/2016/FoodLossConf/02_MaryamRez (2017). Accessed 24 Mar 2020
Bostanci, H., Ok, S., Yilmaz, E.: Valorization of Capia pepper seeds flour-I: spreadable new products development. Waste Biomass Valoriz. (2019). https://doi.org/10.1007/s12649-017-0139-z
Rodriguez-Solana, R., Salgado, J.M., Domínguez, J.M., Cortés-Diéguez, S.: Comparison of Soxhlet, accelerated solvent and supercritical fluid extraction techniques for volatile (GC-MS and GC/FID) and phenolic compounds (HPLC-ESI/MS/MS) from Lamiaceae species. Phytochem. Anal. (2015). https://doi.org/10.1002/pca.2537
Bannon, C.D., Craske, J.D., Hai, N.T., Harper, N., O´Rourke, K.L.: Analysis of fatty acid methyl esters with high accuracy and reliability II. Methylation of fats and oils with boron trifluoride-methanol. J. Chromatogr. A (1982). https://doi.org/10.1016/S0021-9673(00)84856-6
Lavelli, V.: Comparison of the antioxidant activities of extra virgin olive oils. J. Agric. Food Chem. (2002). https://doi.org/10.1021/jf020749o
Nurmi, K., Ossipov, V., Haukioja, E., Pihlaja, K.: Variation of total phenolic content and individual low-molecular-weight phenolics in foliage of mountain birch trees (Betulapubescenss sp. tortuosa). J. Chem. Ecol. (1996). https://doi.org/10.1007/BF02040093
Pellegrini, N., Serafini, M., Colombi, B., Del Rio, D., Salvatore, S., Bianchi, M., Brighenti, F.: Total antioxidants capacity of plants food, beverages and oils consumed in Italy assessed by three different in vitro assays. J. Nutr. (2003). https://doi.org/10.1093/jn/133.9.2812
Cardador-Martínez, A., Albores, A., Bah, M., Calderón-Salinas, V., Castaño-Tostado, E., Guevara-González, R., Shimada-Miyasaka, A., Loarca-Piña, G.: Relationship among antimutagenic, antioxidant and enzymatic activities of methanolic extract from common beans (Phaseolus vulgaris L). Plants Foods Hum. Nutr. (2006). https://doi.org/10.1007/s11130-006-0026-4
Prior, R.L., Gu, X., Bacchiocca, L., Howard, M., Hampsch-Woodill, D., Huang, B., Ou, R.J.: Assay for hydrophilic and lipophilic antioxidant capacity (Oxygen Radical Absorbance Capacity ORAC-FL) of plasma and other biological and food samples. J. Agric. Food Chem. (2003). https://doi.org/10.1021/jf0262256
Kurilich, A.C., Juvik, J.A.: Quantification of carotenoid tocopherol antioxidants in Zea mays. J. Agric. Food Chem. (1999). https://doi.org/10.1021/jf981029d
Cerretani, L., Lerma-García, M.J., Herrero-Martínez, J.M., Gallina-Toschi, T., Simó-Alfonso, E.F.: Determination of tocopherols and tocotrienols in vegetable oils by nanoliquid chromatography with ultraviolet-visible detection using a silica monolithic column. J. Agric. Food Chem. (2010). https://doi.org/10.1021/jf9031537
Qin, C., Yu, C., Shen, Y., Fang, X., Chen, L., Min, J., et al.: Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. Proc. Natl. Acad. Sci. U.S.A. (2014). https://doi.org/10.1073/pnas.140097375111
Gu, L.-B., Pang, H.-L., Lu, K.-K., Liu, H.-M., Wang, X.-D., Qin, G.-Y.: Process optimization and characterization of fragant oil from red pepper (Capsicum annuum L.) seed extracted by subcritical butane extraction. J. Sci. Food Agric. (2017). https://doi.org/10.1002/jsfa.7992
Becerra-Herrera, M., Vélez-Martín, A., Ramos-Merchante, A., Ritcher, P., Beltrán, R., Sayago, A.: Characterization and evaluation of phenolic profiles and color as potential discriminating features among Spanish extra virgin olive oils with protected designation of origin. Food Chem. (2018). https://doi.org/10.1016/j.foodchem.2017.08.106
de Souza Sora, G.T., Pereira Souza, A.H., Ferreira Zielinski, A.A., Isidoro Haminiuk, C.W., Matsushita, M., Peralta, R.M.: Fatty acid composition of Capsicum genus peppers. Ciȇnc Agrotec. (2015). https://doi.org/10.1590/S1413-70542015000400008
Kozƚowska, M., Gruczyńska, E., Ścibisz, I., Rudzińska, : Fatty acids and sterols composition, and antioxidant activity of oils extracted from plant seeds. Food Chem. (2016). https://doi.org/10.1016/j.foodchem.2016.06.102
Wen Chang, N., Chao Huang, P.: Comparative effects of polyunsaturated- to saturated fatty acid ratio versus polyunsaturated- and monounsaturated fatty acids to saturated fatty acid ratio on lipid metabolism in rats. Atherosclerosis (1999). https://doi.org/10.1016/s0021-9150(98)00236-6
Miller, M., Sorkin, J.D., Mastella, L., Sutherland, A., Rhyne, J., Donnelly, P., et al.: Poly is more effective than monounsaturated fat for dietary management in the metabolic syndrome: the muffin study. J. Clin. Lipidol. (2016). https://doi.org/10.1016/j.jacl.2016.04.011
Bersch-Ferreira, A.C., Rodrigues Sampaio, G., Omena Gehringer, M., Ferraz da Silva Torres, E.A., Ross-Fernandez, M.B., Terezada Silva, J., et al.: Association between plasma fatty acids and inflammatory markers in patient with and without insulin resistance and in secondary prevention of cardiovascular disease, a cross-sectional study. Nutr. J. (2018). https://doi.org/10.1186/s12937-018-0342-1
Marangoni, F., Agostoni, C., Borghi, C., Catapano, A.L., Cena, H., Ghiselli, A., et al.: Dietary linoleic acid and human health: focus on cardiovascular and cardiometabolic effects. Atherosclerosis (2020). https://doi.org/10.1016/j.atherosclerosis.2019.11.018
Kamarudin, A.A., Mohd. Esa, N., Saad, N., Sayuti, N.H., Razak, N.A.A.: Heat assisted extraction of phenolic compounds from Eleutherine bulbosa (Mill.) bulb and its bioactive profile using response surface methodology. Ind. Crop Prod. (2020). https://doi.org/10.1016/j.indcrop.2019.112064
Li, M., Chen, X., Deng, J., Ouyang, D., Wang, D., Liang, Y., et al.: Effect of thermal processing on free and bound phenolic compounds and antioxidant activities of hawthorn. Food Chem. (2020). https://doi.org/10.1016/j.foodchem.2020.127429
Lama-Muñoz, A., Contreras, M.M., Espínola, F., Moya, M., Romero, I., Castro, E.: Content of phenolic compounds and manitol in olive leaves extracts from six Spanish cultivars: extraction with the Soxhlet method and pressurized liquids. Food Chem. (2020). https://doi.org/10.1016/j.foodchem.2020.126626
Tuberoso, C.I.G., Jerkowić, I., Maldini, M., Serreli, G.: Phenolic compounds, antioxidant activity, and other characteristics of extra virgin olive oils from Italian autochtonous varieties Tonda di Vilacidro, Tonda di Cagliari, Semidana, and Bosana. J. Chem. (2018). https://doi.org/10.1155/2016/8462741
Wahyuni, Y., Ballester, A.R., Sudarmonowati, E., Bino, R., Bovy, A.: Metabolite biodiversity in pepper (Capsicum) fruit of thirty-two diverse accessions: variation in health-related compounds and implications for breeding. Phytochemistry (2011). https://doi.org/10.1016/j.phytochem.2011.03.016
Provesi, J.G., Dias, C.O., Amante, E.R.: Changes in carotenoids during processing and storage of pumpkin puree. Food Chem. (2011). https://doi.org/10.1016/j.foodchem.2011.03.027
Strati, I.F., Oreopoulou, V.: Recovery of carotenoids from tomato processing by-products- a review. Food Res. Int. (2014). https://doi.org/10.1016/j.foodres.2014.09.032
Chuyen, H.V., Roach, R.D., Golding, J.B., Parks, S.E., Nguyen, M.H.: Encapsulation of carotenoid-rich oil from Gac peel: optimisation of the encapsulation process using a spray drier and the storage stability of encapsulated powder. Powder Technol. (2019). https://doi.org/10.1016/j.powtec.2018.12.012
Run-Yang, Z., Hua-Min, L., Yu-Xiang, M., Xue-De, W.: Characterization of fragrant oil extracted from pepper seed during subcritical propane extraction. LWT-Food Sci. Technol. (2019). https://doi.org/10.1016/j.lwt.2019.04.072
Mailer, R.J., Ayton, J., Graham, K.: The influence of growing region, cultivar and harvest timing on the diversity of Australian olive oil. J. Am. Oil Chem. Soc. (2010). https://doi.org/10.1007/s11746-010-1608-8
de Aguiar, A.C., Sales, L.P., Coutinho, J.P., Barbero, G.F., Godoy, H.T., Martínez, J.: Supercritical carbon dioxide extraction of Capsicum peppers: global yield and capsaicinoid content. J. Supercrit. Fluids (2013). https://doi.org/10.1016/j.supflu.2013.05.008
Materska, M., Perucka, I.: Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). J. Agric. Food Chem. (2005). https://doi.org/10.1021/jf035331k
Xie, J., Yao, S., Ming, J., Deng, L., Zeng, K.: Variations in chlorophyll and carotenoid content and expression of genes involved in pigment metabolism responses to oleocellosis in citrus fruits. Food Chem. (2019). https://doi.org/10.1016/j.foodchem.2018.08.020
Serratosa, M., Lopez-Toledano, A., Merida, J., Medina, M.: Changes in color and phenolic compounds during the raising of grape Cv. Pedro Ximenez. J. Agric. Food Chem. (2008). https://doi.org/10.1021/jf073278k
Acknowledgements
The authors wish to thank La Costeña® for provide the Jalapeño pepper by-products and the fresh Jalapeño fruits, and to Dr. Mario Bueno for technical support. Sandoval-Castro, C. J. wants to thank to National Council of Science and Technology (CONACyT) Mexico for the Master scholarship (366832) awarded.
Funding
The Research and Postgraduate Secretariat of the National Polytechnic Institute, support this work under the following Financed Projects: SIP-20150897, SIP-20164874, and SIP-20172264.
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Valdez-Morales, M., Sandoval-Castro, C.J., Gutiérrez-Dorado, R. et al. Oil Recovery from Jalapeño Pepper By-Products and Analysis of the Industrial Scalding Process on Its Nutraceutical Potential. Waste Biomass Valor 12, 4475–4487 (2021). https://doi.org/10.1007/s12649-020-01323-8
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DOI: https://doi.org/10.1007/s12649-020-01323-8