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Seasonal changes in kinetic parameters of trypsin in gastric and agastric fish

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Abstract

The objective of the present study was to assess if trypsin, a key enzyme involved in protein digestion, presents some kind of functional adaptations to seasonal changes in water temperature in freshwater fish. In order to test this hypothesis, individuals of two fish species Carassius gibelio (agastric) and Perca fluviatilis (gastric) were sampled in the basin of Chany Lake (Siberia, Russia) at two different seasons (spring and summer). Apparent kinetic parameters (Km and Vmax) were determined for both species and seasons at the actual pH values in fish guts, and at actual temperatures. Results showed a significant effect of both the species and sampling season on the apparent kinetic parameters of trypsin. In the case of Prussian carp, Km and Vmax were lower for each assayed temperature (for 5 and 15 °C the differences were significant) for fish sampled in summer when compared to those sampled in spring. In contrast, values of Km in perch tended to be lower in spring at 5 and 25 °C but these differences were not significant, while Vmax showed a significant decrease in summer samples. This suggests a sort of functional adaptation of the same trypsin enzymes to seasonal changes, oriented to maximize protein digestion under variable conditions.

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Funding

This research was supported by the Russian Foundation for Basic Research (grant number 18–34-20122).

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Authors and Affiliations

  1. Institute of Systematics and Ecology of Animals, Siberian Branch of RAS, Frunze Street, 11, Novosibirsk, Russia, 630091

    Mikhail M. Solovyev & Elena N. Kashinskaya

  2. Tomsk State University, Tomsk, Russia, 634050

    Mikhail M. Solovyev

  3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, GSP-7, Miklukho-Maklaya Street, 16/1, Moscow, Russia, 117997

    Eugene A. Rogozhin

  4. Institute of Ecological and Agricultural Biology (X-Bio), Tyumen State University, Volodarskogo Street, 6, Tyumen, Russia, 625003

    Eugene A. Rogozhin

  5. Departamento de Biología y Geología, Facultad de Ciencias, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Almería, La Cañada de San Urbano, 04120, Almería, Spain

    Francisco J. Moyano

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  1. Mikhail M. Solovyev
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  2. Elena N. Kashinskaya
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  4. Francisco J. Moyano
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Contributions

Mikhail M. Solovyev—designing the experiment, analyzing the samples and data, preparing the figures and tables, and writing and revising the manuscript.

Elena N. Kashinskaya—collecting the samples and writing and revising the manuscript.

Eugene A. Rogozhin—writing and revising the manuscript.

Francisco J. Moyano—designing the experiment, analyzing the data, preparing the figures and tables, and writing and revising the manuscript.

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Correspondence to Mikhail M. Solovyev.

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The present research has met the requirements guided by the order of the High and Middle Education Ministry (care for vertebrate animal included in scientific experiments, № 742 from 13.11.1984) and additionally by the Federal Law of the Russian Federation № 498 FL (from 19.12.2018) with regard to the humane treatment of animals.

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Solovyev, M.M., Kashinskaya, E.N., Rogozhin, E.A. et al. Seasonal changes in kinetic parameters of trypsin in gastric and agastric fish. Fish Physiol Biochem 47, 381–391 (2021). https://doi.org/10.1007/s10695-020-00919-0

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