Abstract
It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is developed for the sensitive protein detection and also can be used for the detection of pathogens. PLA employs aptamer or monoclonal/polyclonal antibody-nucleic acid complexes as proximity probes. When the paired proximity probes bind to the same target protein or protein complex, they will be adjacent to each other and form an amplifiable DNA sequence through ligation. Combining the specificity of enzyme-linked immunosorbent assay (ELISA) and sensitivity of polymerase chain reaction (PCR), PLA transforms the detection of protein into the detection of DNA nucleic acid sequence. Therefore, as an ultrasensitive protein assay, PLA has great potential for quantification, localization of protein, and clinical diagnostics. In this review, we summarize the basic principles of PLA and its applications in pathogen detection.
Key Points
• Different forms of proximity ligation assay are introduced.
• Applications of proximity ligation assay in pathogen detection are summarized.
• Proximity ligation assay is an ultrasensitive method to quantify protein and pathogen.
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This study was supported by grants No. 2016YFD0500905 from the National Key Research and Development Program of China, AA18118050 from Science and Technology Major Project of Guangxi, BE2017342 from the Science and Technology Department of Jiangsu Province, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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PZW and YY designed the concept of the review article. PZW wrote the manuscript, YY drawn the figures and wrote the manuscript, and GQZ and TQH critically read and corrected the manuscript. All authors read and approved the final manuscript.
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Wang, P., Yang, Y., Hong, T. et al. Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection. Appl Microbiol Biotechnol 105, 923–935 (2021). https://doi.org/10.1007/s00253-020-11049-1
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DOI: https://doi.org/10.1007/s00253-020-11049-1