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Overexpression of BnGA2ox2, a rapeseed gibberellin 2-oxidase, causes dwarfism and increased chlorophyll and anthocyanin accumulation in Arabidopsis and rapeseed

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Abstract

Plant height is one of the important agronomic traits of crops. Gibberellins (GAs) play an important role in the regulation of plant height. Gibberellin 2-oxidase (GA2ox) inactivates active GAs or their precursors and plays a key role in the catabolism of GAs. Here, we isolated and characterized a fragment from rapeseed (Brassica napus L.), designated BnGA2ox2, that is highly expressed in the silique and flower. Overexpression of BnGA2ox2 in Arabidopsis resulted in GA-deficient phenotypes, including a short hypocotyl, reduced plant height, increased chlorophyll and anthocyanin accumulation, late flowering, and partial sterility. Similarly, when BnGA2ox2 was overexpressed in rapeseed, the transgenic plants had a dwarf phenotype with dark-green leaves and increased chlorophyll and anthocyanin accumulation. In addition, similar dwarf phenotypes were observed in Arabidopsis overexpressing AtGA2ox2. Genetic analysis showed that the atga2ox2 mutation could partially rescue the phenotype of BnGA2ox2 transgenic Arabidopsis plant, indicating that BnGA2ox2 and AtGA2ox2 have conserved functions in vegetative plant growth and stem elongation. These results suggest that BnGA2ox2 regulates stem elongation and chlorophyll and anthocyanin accumulation in rapeseed and Arabidopsis. Therefore, BnGA2ox2 is a new candidate gene for breeding dwarf varieties of rapeseed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31171176), Natural Science Foundation of Hunan province (No. 2018JJ3036), the Basic Research Program of Shenzhen Municipal Science and Technology Innovation Committee (No. JCYJ20170818112212721), and the Basic Research Program of Changsha Municipal Science and Technology (No. kq1901028).

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Author notes

  1. Jindong Yan, Fujiang Xiang, and Piao Yang contributed equally to this work.

Authors and Affiliations

  1. College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China

    Jindong Yan, Fujiang Xiang, Piao Yang, Xin Li, Ming Zhong, Reqing He, Xinmei Li, Xuanming Liu & Xiaoying Zhao

  2. Shenzhen Institute, Hunan University, Shenzhen, 518057, China

    Jindong Yan, Fujiang Xiang, Piao Yang, Xin Li, Ming Zhong, Reqing He, Xinmei Li & Xiaoying Zhao

  3. Yuan Long Ping Agricultural High-tech Co. Ltd, Changsha, 410001, Hunan, China

    Wusheng Peng

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  1. Jindong Yan
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  4. Xin Li
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Contributions

All authors read and approved the final manuscript. XL and XZ contributed to the design and conception of the study, data collection and analysis, and write up of the final paper. JY, FX and PY participated in all phases of the study, experimental design, data collection, analysis and write up the first draft of the paper. XL, MZ, RH, XL, WP provided technical assistance to JY, FX and PY.

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Correspondence to Xuanming Liu or Xiaoying Zhao.

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Yan, J., Xiang, F., Yang, P. et al. Overexpression of BnGA2ox2, a rapeseed gibberellin 2-oxidase, causes dwarfism and increased chlorophyll and anthocyanin accumulation in Arabidopsis and rapeseed. Plant Growth Regul 93, 65–77 (2021). https://doi.org/10.1007/s10725-020-00665-6

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