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Effects of Pesticides on the Diversity of Endophytic Fungi in Tea Plants

  • Environmental Microbiology
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Abstract

We examined the effects of agrochemicals on the endophytic fungal community associated with tea plants. Endophytic fungi were isolated from four different tea plant tissues (bark, xylem, old leaves, new leaves) collected from pesticide-treated and untreated plots. In pesticide-treated plot, the acaricides, fungicides, and insecticides are typically applied 3 times each year. The infection rate was slightly lower in the pesticide-treated plot, but the difference between plots was not statistically significant. Colletotrichum camelliae, Phyllosticta capitalensis, and Pleosporales sp. were common endophytes in both plots. Among a total of 41 fungal species, only 21 were considered common endophytes. Colletotrichum pseudomajus was the predominant endophyte in the bark tissue in the untreated plot, whereas C. camelliae was predominant in the pesticide-treated plot. Paraphaeosphaeria neglecta and Phoma bellendis were predominant in the xylem tissues of samples from the untreated and treated plots, respectively. Colletotrichum camelliae was the most commonly found species in leaf tissues in both plots, but the colonization frequency was significantly lower in the pesticide-treated plot. Species richness was not affected by pesticide treatment. The community structure of endophytic fungi in stem tissues (bark and xylem) differed significantly between plots, but leaf tissue endophytic fungal community structure was not significantly influenced by pesticide treatment.

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Data Availability

Data supporting the findings of this study are available from the corresponding author upon request.

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Acknowledgments

We would like to express our gratitude to Mr. Atsushi Takahashi and the staff of the Saitama Tea Research Institute for providing tea plant materials for this research.

Funding

This research was supported by the Asian Development Bank-Japanese Scholarship Program (ADB-JSP).

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Authors and Affiliations

  1. Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Phyu Mar Win, Emi Matsumura & Kenji Fukuda

  2. Department of Plant Pathology, Yezin Agricultural University, Yezin, Nay Pyi Taw, Myanmar

    Phyu Mar Win

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  1. Phyu Mar Win
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  2. Emi Matsumura
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  3. Kenji Fukuda
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Contributions

All authors contributed to the study conception and design. Sample collection was performed by all authors. Laboratory work and data analysis were conducted by Phyu Mar Win and Emi Matsumura. The first draft of the manuscript was written by Phyu Mar Win and all authors reviewed and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Phyu Mar Win.

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Win, P.M., Matsumura, E. & Fukuda, K. Effects of Pesticides on the Diversity of Endophytic Fungi in Tea Plants. Microb Ecol 82, 62–72 (2021). https://doi.org/10.1007/s00248-020-01675-7

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