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Impacts of Titanium Dioxide Nanoparticles on Serum Parameters and Enzyme Activities of Clarias gariepinus

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Abstract

Impacts of titanium dioxide nanoparticles (TiO2 NPs) on serum glucose, albumin, total protein, cholesterol, triglyceride, sodium (Na+), potassium (K+), and chloride ion (Cl−) levels and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and cholinesterase (ChE) activities of Clarias gariepinus were determined after exposing the fish to 1, 5 and 10 mg/L titanium applied as TiO2 over 1, 4 and 7 days. No mortality was observed during the experiments. Serum glucose and albumin levels increased while serum total protein, cholesterol and triglyceride levels decreased compared to control at the end of the 7th day. Serum Na+, K+ and Cl− levels of C. gariepinus decreased after 7 days of exposure to 10 mg/L TiO2 NPs. ChE activity decreased and AST, ALT, ALP activities increased at all exposure concentrations and LDH activities increased compared to control after 4 and 7 days of exposure to TiO2 NPs in C. gariepinus.

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References

  • Abdel-Khalek AA, Badran SR, Marie MS (2020) The effective adsorbent capacity of rice husk to iron and aluminum oxides nanoparticles using Oreochromis niloticus as a bioindicator: biochemical and oxidative stress biomarkers. Environ Sci Pollut Res 27:23159–23171

    Article  CAS  Google Scholar 

  • Ahn S, Ardoña HAM, Lind JU et al (2018) Mussel-inspired 3D fiber scaffolds for heart-on-a-chip toxicity studies of engineered nanomaterials. Anal Bioanal Chem 410:6141–6154

    Article  CAS  Google Scholar 

  • Al-Bairuty GA, Shaw BJ, Handy RD, Henry TB (2013) Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 126:104–115

    Article  CAS  Google Scholar 

  • Artacho P, Soto-Gamboa M, Verdugo C, Nespolo RF (2007) Blood biochemistry reveals malnutrition in black- necked swans (Cygnus melanocoryphus) living in a conservation priority area. Comp Biochem Physiol A 146:283–290

    Article  Google Scholar 

  • Aschberger K, Micheletti C, Sokull-Kluttgen B, Christensen FM (2011) Analysis of currently available data for characterising the risk of engineered nanomaterials to the environment and human health lessons learned from four case studies. Environ Int 37(6):1143–1156

    Article  CAS  Google Scholar 

  • Bernet D, Schmidt H, Wahli T, Burkhardt-Holm P (2001) Effluent from a sewage treatment works causes changes in serum chemistry of brown trout (Salmo trutta L.). Ecotoxicol Environ Saf 48(2):140–147

    Article  CAS  Google Scholar 

  • Brown JA (1993) Endocrine RESPONSES TO ENVIRONMENTAL POLLUTANTS. In: Rankin JC, Jensen FB (eds) Fish ecophysiology. Chapman and Hall, London, pp 276–296

    Chapter  Google Scholar 

  • Cicik B, Engin K (2005) The effects of cadmium on levels of glucose in serum and glycogen reserves in the liver and muscle tissues of Cyprinus carpio (L., 1758). Turk J Vet Anim Sci 29:113–117

    CAS  Google Scholar 

  • Cowey CB, Walton MJ (1988) Intermediary metabolism in fish nutrition, 2nd edn. Academic Press, San Diego

    Google Scholar 

  • Dange AD (1986) Changes in carbohydrate metabolism in Tilapia Oreochromis mossambicus, during short-term exposure to different types of pollutants. Environ Pollut 41:165–177

    Article  CAS  Google Scholar 

  • Dawood MAO, Koshio S, Ishikawa M, Yokoyama S, El Basuini MF, Hossain MS, Nhu TH, Dossoua S, Moss AS (2016) Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, Gut Microbiota and immune responses of Red Sea Bream, Pagrus major. Fish Shellfish Immunol 49:275–285

    Article  CAS  Google Scholar 

  • Dube PN, Shwetha A, Hosetti BB (2014) Impact of copper cyanide on the key metabolic enzymes of freshwater fish Catla catla (Hamilton). Biotechnol Anim Husb 30:499–508

    Article  Google Scholar 

  • Dutta HM, Haghighi AZ (1986) Methylmercuric chloride and serum cholesterol levels in Blugill Lepomis macrochirus. Bull Environ Contam Toxicol 36:181–185

    Article  CAS  Google Scholar 

  • Eddy FB (1982) Osmotic and ıonic regulation in captive fish with particular reference to Salmonids. Comp Biochem Physiol 73(B):125–141

    Google Scholar 

  • Federici G, Shaw BJ, Handy RD (2007) Toxicity of titanium dioxide nanoparticles to rainbow trout (Oncorhynchus mykiss): gill ınjury, oxidative stress, and other physiological effects. Aquat Toxicol 84(4):415–430

    Article  CAS  Google Scholar 

  • Fırat Ö, Kargın F (2010a) Biochemical Alterations Induced by Zn and Cd Individually or in Combination in the Serum of Oreochromis niloticus. Fish Physiol Biochem 36:647–653

    Article  Google Scholar 

  • Fırat Ö, Kargın F (2010b) Individual and combined effects of heavy metals on serum biochemistry of Nile Tilapia Oreochromis niloticus. Arch Environ Contam Toxicol 58:151–157

    Article  Google Scholar 

  • Hao L, Wang Z, Xing B (2009) Effect of sub-acute exposure to TiO2 nanoparticles on oxidative stress and histopathological changes in Juvenile Carp (Cyprinus carpio). J Environ Sci 21:1459–1466

    Article  CAS  Google Scholar 

  • Harper AF, Rodwell VW, Mayes PA (1979) Review of physiological chemistry, 17th edn. Lang. Medical, Los Atlos, p 9422

    Google Scholar 

  • Heath AG (1995) Water pollution and fish physiology. CRC Press, Florida, USA, p 245

    Google Scholar 

  • Hoseini SM, Hosseini SA, Soudagar M (2012) Dietary tryptophan changes serum stress markers, enzyme activity, and ions concentration of wild common carp Cyprinus carpio exposed to ambient copper. Fish Physiol Biochem 38(5):1419–1426

    Article  CAS  Google Scholar 

  • Ijadpanah-Saravy H, Safari M, Darban-Khodadadi A, Razaei A (2014) Synthesis of titanium dioxide nanoparticles for photocatalytic degradation of cyanide in wastewater. Anal Lett 47(10):1772–1782

    Article  CAS  Google Scholar 

  • Iwama GK, Vijayan MM, Forsyth RB, Ackerman PA (1999) Heat shock proteins and physiological stress in fish. Am Zool 39:901–909

    Article  CAS  Google Scholar 

  • Jiraungkoorskul W, Upatham ES, Kruatrachue M, Shaphong S, Vichasri-Grams S, Pokethitiyook P (2003) Biochemical and histopathological effects of glyphosate herbicide on Nile Tilapia (Oreochromis niloticus). Environ Toxicol 18:260–267

    Article  CAS  Google Scholar 

  • Karan V, Vitorović S, Tutundžić V, Poleksić V (1998) Functional enzymes activity and gill histology of carp after copper sulfate exposure and recovery. Ecotoxicol Environ Saf 40(1):49–55

    Article  CAS  Google Scholar 

  • Kuşatan Z, Cicik B (2004) Clarias lazera (Valenciennes, 1840)’da Kadmiyum’un Solungaç, Karaciğer, Böbrek, Dalak ve Kas Dokularındaki Birikimi. Eğirdir Su Ürünleri Fakültesi Dergisi 2(12):59–66

    Google Scholar 

  • Lee H, Choi W (2002) Photocatalytic oxidation of arsenite in TiO2 suspension: kinetics and mechanisms. Environ Sci Technol 36(17):3872–3878

    Article  CAS  Google Scholar 

  • Levesque HM, Moon TW, Campbell PGC, Hontela A (2002) Seasonal variation in carbohydrate and lipid metabolism of Yellow Perch (Perca flavescens) chronically exposed to metals in the field. Aquat Toxicol 60:257–267

    Article  CAS  Google Scholar 

  • Lewis SD, Lewis WM (1971) The effects of zinc, copper on the osmolarity of blood serum of the channel catfish, Ictalurus punctatus Rafinesque and Golden Shiner, Notemigomus crysoleucas Mitchell. Trans Am Fish Soc 100:639–643

    Article  CAS  Google Scholar 

  • Nel AE, Madler L, Velegol D, Xia T, Hoek EM, Somasundaran P, Klaessig F, Castranova V, Thompson M (2009) Understanding biophysiochemical interactions at the nanobio interface. Nat Mater 8:543–557

    Article  CAS  Google Scholar 

  • Pelaez M, Nolan NT, Pillai SC et al (2012) A review on the visible light active titanium dioxide photocatalysts for environmental applications. Appl Catal B 125:331–349

    Article  CAS  Google Scholar 

  • Prashanth MS (2012) Acute toxicity, behavioral and nitrogen metabolism changes of sodium cyanide affected on tissues of Tilapia mossambica (Perters). Drug Chem Toxicol 35:178–183

    Article  CAS  Google Scholar 

  • Ramesh M, Senthil Kumaran S, Kavith C, Saravanan M, Mustafa A (2007) Primary stress responses of common carp, Cyprinus carpio exposed to copper toxicity. Acta Ichthyol Piscat 37:81–85

    Article  Google Scholar 

  • Schaperclaus W, Kulow H, Schreckenbach K (1992) In: Fish diseases, Vol. 1. Rotterdam, Septicemia of Fish, Fish Disease Leaflet, 68:1–24.

  • Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF (2010) Metal-based nanoparticles and their toxicity assessment. Wiley Interdisc Rev Nanomed Nanobiotechnol 2(5):544–568

    Article  CAS  Google Scholar 

  • Shaheen T, Akhtar T (2012) Assessment of chromium toxicity in Cyprinus carpio through hematological and biochemical blood markers. Turk J Zool 36:682–690

    CAS  Google Scholar 

  • Suresh A, Sivaramakrishna B, Radhakrishnaiah K (1995) Cadmium ınduced changes in ıon levels and ATPase activities in the muscle of the fry and fingerlings of the freshwater fish, Cyprinus carpio. Chemosphere 30(2):365–375

    Article  Google Scholar 

  • Tunçsoy M, Duran S, Yesilbudak B, Ay O, Cicik B, Erdem C (2016) Short term effects of zınc on some sera bıochemıcal parameters and on tıssue accumulatıon of Clarias gariepinus. Fresenius. Environ Bull 25(2):658–664

    Google Scholar 

  • Vaglio A, Landriscina C (1999) Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicol Environ Saf 43:111–116

    Article  CAS  Google Scholar 

  • Vosyliene MZ (1999) The effect of heavy metals on hematological ındices. Acta Zool Litvanica Hydrobiol 9:76–82

    Article  Google Scholar 

  • Weir A, Westerhoff P, Fabricius L, Hristovski K, von Goetz N (2012) Titanium dioxide nanoparticles in food and personal care products. Environ Sci Technol 46(4):2242–2250

    Article  CAS  Google Scholar 

  • Wu RS, Pollino CA, Au DW, Zheng DW, Yuen B, Lam PK (2003) Evaluation of biomarkers of exposure and effect in juvenile areolated grouper (Epinephelus areolatus) on food-borne exposure to benzo-a- pyrene. Environ Toxicol Chem 22:68–73

    Article  Google Scholar 

  • Yang JL, Chen HC (2003) Effects of gallium on common carp (Cyprinus carpio): acute test, serum biochemistry, and erythrocyte morphology. Chemosphere 53:877–882

    Article  CAS  Google Scholar 

  • Yousef MI, Awad TI, Elhag FA, Khaled FA (2007) Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits. Toxicology 235:194–202

    Article  CAS  Google Scholar 

Download references

Acknowledgements

I would like to acknowledge Prof.Dr. Cahit ERDEM and Dr. Servet DURAN from Cukurova University, Science and Letters Faculty, Biology Department. I also would like to acknowledge Prof.Dr. Philip DEMOKRITOU and Dr. Georgios PYRGIOTAKIS from Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University.

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  1. Faculty of Science and Letters, Biology Department, Çukurova University, Adana, Turkey

    Mustafa Tunçsoy

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Correspondence to Mustafa Tunçsoy.

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Tunçsoy, M. Impacts of Titanium Dioxide Nanoparticles on Serum Parameters and Enzyme Activities of Clarias gariepinus. Bull Environ Contam Toxicol 106, 629–636 (2021). https://doi.org/10.1007/s00128-020-03100-8

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