Abstract
Biolog EcoPlates are useful tools to study the metabolic function of microbial community in water bodies; however, the influence of aquaculture water, which contains complex chemical compositions, on color development in Biolog EcoPlates, has not been clearly illustrated. Previously, we detected a delayed color development in original water samples, comparing to that in the tenfold diluted samples. The objective of this study was to evaluate the differences of average well color development (AWCD) and community-level physiological profiles (CLPP) between the original and tenfold diluted water samples collected from three intensive Litopenaeus vannamei culture ponds, and to explore potential causes of the delayed color development by measuring microbial community structure and physiochemical indexes. Compared with the tenfold diluted samples, the original water samples from all three ponds exhibited delayed color development and reduced microbial metabolic activity. The original water samples exhibited higher utilization of carbohydrates, while significantly higher utilization of amino acids was observed in the corresponding diluted samples. The CLPP of the original water samples was significantly correlated with the microbial community structure at 48 h of incubation, meanwhile both CLPP and community structure were substantially influenced by NO3–N and NO2–N. Therefore, NO2-N and NO3-N accumulation in the intensive Litopenaeus vannamei culture pond might be responsible for the delayed color development in Biolog EcoPlates. In order to minimize the influence of aquaculture water on color development in Biolog EcoPlates, a shorter incubation period is recommended.
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Data availability
The raw sequence data from this study were submitted to the National Center for Biotechnology Information Sequence Read Archive under accession number SRR9157350-SRR9157364 (BioProject ID: PRJNA544379).
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Acknowledgments
We thank Professor Qian Yang and Professor Wan-cong Yu for their valuable comments and suggestions for improving this manuscript. This research was supported by the Open Fund of Ministry of Education Key Laboratory of Molecular Microbiology and Technology of Nankai University, the Special Foundation of President of Tianjin Academy of Agricultural Sciences (16015), and the Innovative Research and Experimental Projects for Young Researchers of Tianjin Academy of Agricultural Sciences (2018007). We would also like to thank TopEdit (www.topeditsci.com) for English language editing of this manuscript.
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This research was supported by the Open Fund of Ministry of Education Key Laboratory of Molecular Microbiology and Technology of Nankai University, the Special Foundation of President of Tianjin Academy of Agricultural Sciences (16015), and the Innovative Research and Experimental Projects for Young Researchers of Tianjin Academy of Agricultural Sciences (2018007).
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Qiong Zhao and Fengxing Xie conceived and designed the study. Qiong Zhao, Ke Zhou, Fengfeng Zhang, Fengxing Xie, Haibo Sun, and Yujie Zhao performed the experiments. Qiong Zhao drafted the manuscript. Fengxing Xie and Haibo Sun reviewed and revised the manuscript. All authors read and approved the manuscript.
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Zhao, Q., Zhou, K., Zhang, F. et al. Effect of aquaculture water eutrophication on color development in Biolog EcoPlates. Aquacult Int 29, 373–386 (2021). https://doi.org/10.1007/s10499-020-00632-y
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DOI: https://doi.org/10.1007/s10499-020-00632-y