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Polyphasic Characterization of Plant Growth Promoting Cellulose Degrading Bacteria Isolated from Organic Manures

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Abstract

In the present study, twenty seven cellulose-degrading bacteria (CDB) were isolated from various organic manures and their cellulolytic activities were determined. The bacterial isolate CDB-26 showed the highest cellulolytic index, released 0.507 ± 0.025 mg/ml glucose and produced 0.196 ± 0.014 IU/ml cellulase enzyme under in vitro conditions. Biochemically, all the 27 isolates showed difference in the 6 biochemical tests performed. Further, all the 27 CDB isolates were subjected to various plant growth-promoting activities, and all CDB strains were positive for IAA production, GA3 production and siderophore production, whereas 19 strains were positive for ACC deaminase activity, 21 strains showed NH3 production and 19 strains were positive for HCN production. Out of 27 CDB isolates, 18 isolates were able to solubilize phosphate, 21 isolates were able to solubilize potash and 10 CDB isolates were found positive for silica solubilization. The molecular diversity among different CDB isolates was studied through ARDRA and demonstrated very high genetic diversity among these bacteria. The in vitro cellulose-degradation potential of these CDB isolates using vegetable waste as substrate were also assessed, and the 3 CDB isolates viz. Serratia surfactantfaciens (CDB-26), Stenotrophomonas rhizophila (CDB-16) and Pseudomonas fragi (CDB-5) showed the highest cellulose-degrading potential under in vitro conditions. Hence, the cellulolytic microbes isolated in the present study could be used for effective bioconversion of plant biomasses into enriched compost.

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Abbreviations

PCR–RFLP:

Polymerase chain reaction–restriction fragment length polymorphism

ARDRA:

Amplified 16S ribosomal DNA restriction analysis

UPGMA:

Unweighted pair group method with arithmetic mean

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Acknowledgements

The financial assistance from All India Network Project on soil biodiversity and bio-fertilizers and Rastriya Krishi Vikas Yojana (RKVY) research project are highly acknowledged. All India Network Project on Organic farming is gratefully acknowledged for providing freshly prepared organic manures. The support of Dean, RCA and Director of Research, MPUAT is also acknowledged.

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, India

    Devendra Jain,  Ravina, Ali Asger Bhojiya, Surya Chauhan & Deepak Rajpurohit

  2. Department of Agriculture and Veterinary Sciences, Mewar University, Chittaurgarh, Rajasthan, India

    Ali Asger Bhojiya

  3. Indian Institute of Soil Science, Indian Council of Agricultural Research, Bhopal, Madhya Pradesh, 462038, India

    Santosh R. Mohanty

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  1. Devendra Jain
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  6. Santosh R. Mohanty
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Contributions

DJ, SRM conceived and designed the experiments; R, AAB, SC performed laboratory experiments; DJ and DR wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Devendra Jain.

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Jain, D., Ravina, Bhojiya, A.A. et al. Polyphasic Characterization of Plant Growth Promoting Cellulose Degrading Bacteria Isolated from Organic Manures. Curr Microbiol 78, 739–748 (2021). https://doi.org/10.1007/s00284-020-02342-3

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