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Application of Simultaneously Validated UHPLC-PDA and LC–ESI–MS/MS Methods for Determining 22 Antidepressants and Anxiolytics in Food Matrix Samples

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Abstract

An analytical method based on UHPLC-PDA and LC–ESI–MS/MS was developed for the simultaneous and reliable determination of 22 antidepressant and anti-anxiety pharmaceutical compounds in adulterated functional foods. The method was validated in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), method detection limit (MDL), method quantitation limit (MQL), precision, accuracy, recovery, stability, and matrix effect. For solid and liquid blank samples, the developed method had LOD = 0.10–1.01 μg/mL for UHPLC and 0.01–5.05 ng/mL for LC–ESI–MS/MS, respectively, LOQ = 0.30–3.02 μg/mL and 0.03–15.15 ng/mL, and R2 > 0.999 and R2 > 0.99. Recoveries from spiked blank samples in powder, liquid, tablet, and capsule forms were determined to be 80.13–113.76%. The precision, accuracy, and stability were acceptable, and the matrix effect was insignificant. A total of 118 real samples were successfully analysed using the proposed rapid screening method.

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Acknowledgements

This study was supported by a grant (Number: 18181MFDS421) from the Ministry of Food and Drug Safety of Korea in 2019.

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  1. Nam Sook Kim and Hwan Seong Choi contributed equally to this article.

Authors and Affiliations

  1. Center for Advanced Analysis, Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation, Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 363-700, Republic of Korea

    Nam Sook Kim, Hwan Seong Choi, Na Young Lim, Ji Hyun Lee, Hyungil Kim & Sun Young Baek

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  1. Nam Sook Kim
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  2. Hwan Seong Choi
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Correspondence to Sun Young Baek.

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Kim, N.S., Choi, H.S., Lim, N.Y. et al. Application of Simultaneously Validated UHPLC-PDA and LC–ESI–MS/MS Methods for Determining 22 Antidepressants and Anxiolytics in Food Matrix Samples. Chromatographia 84, 233–247 (2021). https://doi.org/10.1007/s10337-020-04000-z

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