Abstract
Applying manure to temperate agricultural soils in the fall season is often justified by the assumption that mineral nitrogen (N) is stable in frozen soils, although pulses of nitrous oxide (N2O) are emitted when the soil thaws during winter months. Nitrous oxide loss was monitored during three freeze-thaw cycles in agricultural soils that received manure and had a growing cover crop before they were frozen. Soil was mixed with N fertilizer treatments (none, liquid dairy manure, solid dairy manure, or urea) and packed in 0.2-L pots, half of which were planted with an annual ryegrass (Lolium multiflorum Lam.) cover crop. After 3 weeks, pots were transferred to a freezer at − 4 °C, or left in a refrigerator at + 4 °C. Frozen pots were thawed at + 4 °C. Production of N2O was measured after 0, 3, 6, and 9 h of thawing; then the pots were destructively sampled to determine the soil mineral N concentration. The N fertilizer and cover crop treatments did not affect N2O production, and only 14% of the variation in N2O production was explained by soil mineral N concentration. However, there was a 6–9-fold increase in N2O production, relative to soil mineral N, in pots that underwent freeze-thaw cycles compared to pots that were left at + 4 °C. It appears that N2O was produced in frozen soils at − 4 °C, trapped under ice, and subsequently released when the soils thawed at + 4 °C, suggesting that N2O-producing reactions do not stop when manured soils are frozen.
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References
Beaulieu MS (2004) Manure management in Canada, vol 1. Farm Environmental Management in Canada, Ottawa
Cavigelli MA, Parkin TB (2012) Chapter 9 - Cropland management contributions to greenhouse gas flux: Central and Eastern U.S. In: Franzluebbers AJ, Follett RF (eds) Managing Agricultural Greenhouse Gases. Academic Press, San Diego, pp 129–165
Chapuis-Lardy L, Wrage-Mönnig N, Metay A, Chotte J-L, Bernoux M (2007) Soils, a sink for N2O? A review. Global Change Biol 13:1–17
Chen Y, Tessier S, MacKenzie AF, Laverdière MR (1995) Nitrous oxide emission from an agricultural soil subjected to different freeze-thaw cycles. Agric, Ecosyst Environ 55:123–128
Clark K, Chantigny MH, Angers DA, Rochette P, Parent L-É (2009) Nitrogen transformations in cold and frozen agricultural soils following organic amendments. Soil Biol Biochem 41:348–356
Cober JR, Macrae ML, Van Eerd LL (2018) Nutrient release from living and terminated cover crops under variable freeze-thaw cycles. Agron J 110:1036–1045
Congreves KA, Wagner-Riddle C, Si BC, Clough TJ (2018) Nitrous oxide emissions and biogeochemical responses to soil freezing-thawing and drying-wetting. Soil Biol Biochem 117:5–15
DeLuca TH, Keeney DR, McCarty GW (1992) Effect of freeze-thaw events on mineralization of soil nitrogen. Biol Fertil Soils 14:116–120
Eghball B (2000) Nitrogen mineralization from field-applied beef cattle feedlot manure or compost. Soil Sci Soc Am J 64:2024–2030
Eghball B, Wienhold BJ, Gilley JE, Eigenberg RA (2002) Mineralization of manure nutrients. J Soil Water Conserv 57:470–473
Ejack L, Whalen JK, Madramootoo CA (2021) Carbon availability limits the denitrification potential of sandy loam soil from corn agroecosystems with long-term tillage and residue management. Can J Soil Sci 101:5. https://doi.org/10.1139/cjss-2020-0097
Elliott AC, Henry HAL (2009) Freeze-thaw cycle amplitude and freezing rate effects on extractable nitrogen in a temperate old field soil. Biol Fertil Soils 45:469–476
Gao D, Zhang L, Liu J, Peng B, Fan Z, Dai W, Jiang P, Bai E (2018) Responses of terrestrial nitrogen pools and dynamics to different patterns of freeze-thaw cycle: a meta-analysis. Global Change Biol 24:2377–2389
Gillam KM, Zebarth BJ, Burton DL (2008) Nitrous oxide emissions from denitrification and the partitioning of gaseous losses as affected by nitrate and carbon addition and soil aeration. Can J Soil Sci 88:133–143
Guo XB, Drury CF, Yang XM, Reynolds WD, Zhang RD (2011) Influence of current and previous crops on soil basal and potential denitrification rates. Biol Fertil Soils 47:937–947
Han Z, Walter MT, Drinkwater LE (2017) N2O emissions from grain cropping systems: a meta-analysis of the impacts of fertilizer-based and ecologically-based nutrient management strategies. Nutr Cycl Agroecosys 107:335–355
Henry HAL (2007) Soil freeze-thaw cycle experiments: trends, methodological weaknesses and suggested improvements. Soil Biol Biochem 39:977–986
Hu HW, Chen D, He JZ (2015) Microbial regulation of terrestrial nitrous oxide formation: understanding the biological pathways for prediction of emission rates. FEMS Microbiol Rev 39:729–749
Jayasundara S, Wagner-Riddle C, Parkin G, Lauzon J, Fan MZ (2010) Transformations and losses of swine manure 15N as affected by application timing at two contrasting sites. Can J Soil Sci 90:55–73
Kariyapperuma KA, Wagner-Riddle C, Furon AC, Li CS (2011) Assessing spring thaw nitrous oxide fluxes simulated by the DNDC model for agricultural soils. Soil Sci Soc Am J 75:678–690
Kool DM, Dolfing J, Wrage N, Van Groenigen JW (2011) Nitrifier denitrification as a distinct and significant source of nitrous oxide from soil. Soil Biol Biochem 43:174–178
Koponen HT, Flöjt L, Martikainen PJ (2004) Nitrous oxide emissions from agricultural soils at low temperatures: a laboratory microcosm study. Soil Biol Biochem 36:757–766
Li C, Aber J, Stange F, Butterbach-Bahl K, Papen H (2000) A process-oriented model of N2O and NO emissions from forest soils: 1. model development. J Geophys Res-Atmos 105:4369–4384
Li X, Sørensen P, Olesen JE, Petersen SO (2016) Evidence for denitrification as main source of N2O emission from residue-amended soil. Soil Biol Biochem 92:153–160
Ludwig B, Wolf I, Teepe R (2004) Contribution of nitrification and denitrification to the emission of N2O in a freeze-thaw event in an agricultural soil. J Plant Nutr Soil Sci 167:678–684
Morkved PT, Dorsch P, Henriksen TM, Bakken LR (2006) N2O emissions and product ratios of nitrification and denitrification as affected by freezing and thawing. Soil Biol Biochem 38:3411–3420
Nannipieri P, Penton CR, Purahong W, Schloter M, van Elsas JD (2019) Recommendations for soil microbiome analyses. Biol Fertil Soils 55:765–766
Pattey E, Blackburn LG, Strachan IB, Desjardins R, Dow D (2008) Spring thaw and growing season N2O emissions from a field planted with edible peas and a cover crop. Can J Soil Sci 88:241–249
Pelster DE, Chantigny MH, Rochette P, Angers DA, Laganière J, Zebarth B, Goyer C (2013) Crop residue incorporation alters soil nitrous oxide emissions during freeze-thaw cycles. Can J Soil Sci 93:415–425
Pelster DE, Chantigny MH, Rochette P, Bertrand N, Angers D, Zebarth BJ, Goyer C (2019) Rates and intensity of freeze-thaw cycles affect nitrous oxide and carbon dioxide emissions from agricultural soils. Can J Soil Sci 99:472–484
Risk N, Snider D, Wagner-Riddle C (2013) Mechanisms leading to enhanced soil nitrous oxide fluxes induced by freeze-thaw cycles. Can J Soil Sci 93:401–414
SAS Institute I (2013) SAS/ACCESS 9.4. Cary, NC, USA
Sims GK, Ellsworth TR, Mulvaney RL (1995) Microscale determination of inorganic nitrogen in water and soil extracts. Commun Soil Sci Plant Anal 26:303–316
Singer JW, Cambardella CA, Moorman TB (2008) Enhancing nutrient cycling by coupling cover crops with manure injection. Agron J 100:1735–1739
Song Y, Zou YC, Wang GP, Yu XF (2017) Altered soil carbon and nitrogen cycles due to the freeze-thaw effect: a meta-analysis. Soil Biol Biochem 109:35–49
Tatti E, Goyer C, Chantigny M, Wertz S, Zebarth BJ, Burton DL, Filion M (2014) Influences of over winter conditions on denitrification and nitrous oxide-producing microorganism abundance and structure in an agricultural soil amended with different nitrogen sources. Agric, Ecosyst Environ 183:47–59
Thomas BW, Hao XY, Larney FJ, Goyer C, Chantigny MH, Charles A (2017) Non-legume cover crops can increase non-growing season nitrous oxide emissions. Soil Sci Soc Am J 81:189–199
Wagner-Riddle C, Congreves KA, Abalos D, Berg AA, Brown SE, Ambadan JT, Gao XP, Tenuta M (2017) Globally important nitrous oxide emissions from croplands induced by freeze-thaw cycles. Nat Geosci 10:279–283
Wagner-Riddle C, Furon A, McLaughlin NL, Lee I, Barbeau J, Jayasundara S, Parkin G, Von Bertoldi P, Warland J (2007) Intensive measurement of nitrous oxide emissions from a corn-soybean-wheat rotation under two contrasting management systems over 5 years. Global Change Biol 13:1722–1736
Wagner-Riddle C, Hu QC, van Bochove E, Jayasundara S (2008) Linking nitrous oxide flux during spring thaw to nitrate denitrification in the soil profile. Soil Sci Soc Am J 72:908–916
Wu X, Chen Z, Kiese R, Fu J, Gschwendter S, Schloter M, Liu CY, Butterbach-Bahl K, Wolf B, Dannenmann M (2020) Dinitrogen (N2) pulse emissions during freeze-thaw cycles from montane grassland soil. Biol Fertil Soils 56:959–972
Yang JY, Huffman EC, Drury CF, Yang XM, De Jong R (2011) Estimating the impact of manure nitrogen losses on total nitrogen application on agricultural land in Canada. Can J Soil Sci 91:107–122
Acknowledgments
We acknowledge the technical and logistical support provided by Dr. Ian Strachan, Dr. Pierre Dutilleul, Marc Samoisette, Hicham Benslim, Ian Ritchie, Hélène Lalande, and Chih-Yu Hung.
Funding
This research was supported by funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) through the Discovery Grant # RGPIN-2017-05391. The senior author also received financial support from the AgroPhytoSciences program (NSERC CREATE 449133-2014).
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Ejack, L., Whalen, J.K. Freeze-thaw cycles release nitrous oxide produced in frozen agricultural soils. Biol Fertil Soils 57, 389–398 (2021). https://doi.org/10.1007/s00374-020-01537-x
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DOI: https://doi.org/10.1007/s00374-020-01537-x