Abstract
The uses of vegetable oils are determined by functional properties arising from their chemical composition. Soybean oil was previously used in margarines and baked foods after partial hydrogenation to achieve heat and oxidative stability. This process, however, generates trans fats that are now excluded from food use because of cardiovascular health risks. Also present in soybean oil are the anti-oxidant tocopherols, with α-tocopherol (vitamin E) typically present as a minor component compared to γ-tocopherol. Genetic improvement of the fatty acid profile and tocopherol profile is an attractive solution to increase the functional and health qualities of soybean oil. The objective of this research was to develop resources to directly select with molecular markers for the elevated vitamin E trait in soybean oil and to use a molecular breeding approach to combine elevated vitamin E with the high oleic/low linolenic acid seed oil trait that improves oil functionality and nutrition. New soybean germplasm was developed from the molecular breeding strategy that selected for alleles of six targeted genes. Seed oil from the novel soybean germplasm was confirmed to contain increased vitamin E α-tocopherol along with a high oleic acid/low linolenic acid profile.
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Acknowledgments
The authors acknowledge Christine Cole and Paul Little for essential technical assistance for most aspects of field management and gas chromatography phenotyping. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.
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Funding was provided by the USDA/ARS. Support was also provided in part by grants from the Nebraska Soybean Board and the United States Department of Agriculture-National Institute of Food and Agriculture (grant no. 2015-67013-2283) to EBC.
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KB conceived and conducted or directed the experiments except for the tocopherol analyses. JHK conducted the statistical analyses and prepared figures. ARK and EBC conducted the tocopherol analyses and edited the manuscript. KH and KB wrote the manuscript.
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The authors declare no conflicts of interest. Code availability is not applicable. Ethics approval is not applicable. Consent to participate is not applicable. All authors consented for publication. Soybean germplasm is available for research with a Material Transfer Agreement. Support was also provided by grants from the Nebraska Soybean Board and the United States Department of Agriculture-National Institute of Food and Agriculture (grant no. 2015-67013-2283) to EBC.
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Hagely, K., Konda, A.R., Kim, JH. et al. Molecular-assisted breeding for soybean with high oleic/low linolenic acid and elevated vitamin E in the seed oil. Mol Breeding 41, 3 (2021). https://doi.org/10.1007/s11032-020-01184-y
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DOI: https://doi.org/10.1007/s11032-020-01184-y