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Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel

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Abstract

Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity for tyrosine/DOPA-tethered polymeric biomaterials throughout a broad pH range. A dual-barrel syringe with one part containing chitosan/mTyr-CNK solution and the other containing gelatin solution with/without nanohydroxyapatite was successfully used to form homogeneous hydrogels at room temperature followed by 37 °C to simulate an in situ injection approach. The obtained hydrogels exhibited an average pore size greater than 150 µm and high swelling ratios with similar mechanical properties to other chemically crosslinked chitosan/gelatin hydrogels. The in vitro degradation properties and cellular viability suggested that the hydrogels could be used as biodegradable and biocompatible scaffolds for biomedical applications, such as space filling biomaterials and delivery vehicles for bioactive molecules and cells. These results demonstrated that mTyr-CNK-mediated hydrogels have remarkable promise as an injectable scaffold biomaterial.

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References

  1. L. Fan, H. Yang, J. Yang, M. Peng and J. Hu, Carbohyd. Polym., 146, 427 (2016).

    Article  CAS  Google Scholar 

  2. M. Liu, X. Zeng, C. Ma, H. Yi, Z. Ali, X. B. Mou, S. Li, Y. Deng and N. Y. He, Bone Res., 5, 17014 (2017).

    Article  CAS  Google Scholar 

  3. J. Patterson, M. M. Martino and J. A. Hubbell, Mater. Today, 13, 14 (2010).

    Article  CAS  Google Scholar 

  4. Y. L. Li, J. Rodrigues and H. Tomas, Chem. Soc. Rev., 41, 2193 (2012).

    Article  CAS  Google Scholar 

  5. R. LogithKumar, A. KeshavNarayan, S. Dhivya, A. Chawla, S. Saravanan and N. Selvamurugan, Carbohyd. Polym., 151, 172 (2016).

    Article  CAS  Google Scholar 

  6. L. D. Zhu and K. M. Bratlie, Biochem. Eng. J., 132, 38 (2018).

    Article  CAS  Google Scholar 

  7. K. Su and C. M. Wang, Biotechnol. Lett., 37, 2139 (2015).

    Article  CAS  Google Scholar 

  8. Y.J. Yang, Y.S. Choi and H.J. Cha, Biotechnol. J., 13, 1800086 (2018).

    Article  Google Scholar 

  9. L. Cui, J. F. Jia, Y. Guo, Y. Liu and P. Zhu, Carbohyd. Polym., 99, 31 (2014).

    Article  CAS  Google Scholar 

  10. S. M. Saraiva, S. P. Miguel, M. P. Ribeiro, P. Coutinho and I. J. Correia, RSC Adv., 5, 63478 (2015).

    Article  CAS  Google Scholar 

  11. M. Nieto-Suarez, M. A. Lopez-Quintela and M. Lazzari, Carbohyd. Polym., 141, 175 (2016).

    Article  CAS  Google Scholar 

  12. Y. R. Choi, E. H. Kim, S. Lim and Y. S. Choi, Biochem. Eng. J., 129, 50 (2018).

    Article  CAS  Google Scholar 

  13. X. Bai, M. Gao, S. Syed, J. Zhuang, X. Xu and X. Q. Zhang, Bioact. Mater., 3, 401 (2018).

    Article  Google Scholar 

  14. M. Otsuka, Biol. Pharm. Bull., 36, 1653 (2013).

    Article  CAS  Google Scholar 

  15. C. Ohtsuki, M. Kamitakahara and T. Miyazaki, J. R. Soc. Interface., 6, S349 (2009).

    Article  CAS  Google Scholar 

  16. H. Zhou and J. Lee, Acta Biomater., 7, 2769 (2011).

    Article  CAS  Google Scholar 

  17. R. Z. LeGeros, Clin. Mater., 14, 65 (1993).

    Article  CAS  Google Scholar 

  18. S. I. Stupp and G. W. Ciegler, J. Biomed. Mater. Res., 26, 169 (1992).

    Article  CAS  Google Scholar 

  19. H. Do, E. Kang, B. Yang, H.J. Cha and Y.S. Choi, Sci. Rep., 7, 17267 (2017).

    Article  Google Scholar 

  20. R.N. Raja Mohd Hafidz, C.M. Yaakob, I. Amin and A. Noorfaizan, Int. Food Res. J., 18, 787 (2011).

    Google Scholar 

  21. E. H. Kim, S. Lim, T.E. Kim, I.O. Jeon and Y.S. Choi, Biotechnol. Bioprocess Eng., 23, 500 (2018).

    Article  CAS  Google Scholar 

  22. C.Q. Yan and D.J. Pochan, Chem. Soc. Rev., 39, 3528 (2010).

    Article  CAS  Google Scholar 

  23. L. Guo, R.H. Colby, C.P. Lusignan and A.M. Howe, Macromolecules, 36, 10009 (2003).

    Article  CAS  Google Scholar 

  24. S.F. Yan, T.T. Wang, X. Li, Y.H. Jian, K.X. Zhang, G.F. Li and J.B. Yin, RSC Adv., 7, 17005 (2017).

    Article  CAS  Google Scholar 

  25. M.I. Gariboldi and S.M. Best, Front Bioeng. Biotechnol., 3, 151 (2015).

    Article  Google Scholar 

  26. K.S. Anseth, C.N. Bowman and L. Brannon-Peppas, Biomaterials, 17, 1647 (1996).

    Article  CAS  Google Scholar 

  27. H. Jiankang, L. Dichen, L. Yaxiong, Y. Bo, Z. Hanxiang, L. Qin, L. Bingheng and L. Yi, Acta Biomater., 5, 453 (2009).

    Article  Google Scholar 

  28. Y.Y. Pey, D.M. Guo, O.D. An, Z.Y. Xiao, S.R. Zhai and B. Zhai, Korean J. Chem. Eng., 35, 2384 (2018).

    Article  Google Scholar 

  29. S. Jo, S. Park, Y. Oh, J. Hong, H.J. Kim, K.J. Kim, K.K. Oh and S.H. Lee, Biotechnol. Bioprocess. Eng., 24, 145 (2019).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Marine Biomaterials Research Center grant from Marine Biotechnology Program of the Korea Institute of Marine Science & Technology Promotion funded by the Ministry of Oceans and Fisheries, Korea and by the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF- 2020R1I1A3072957).

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Correspondence to Yoo Seong Choi.

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Lim, S., Jeong, D., Ki, MR. et al. Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel. Korean J. Chem. Eng. 38, 98–103 (2021). https://doi.org/10.1007/s11814-020-0672-5

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  • DOI: https://doi.org/10.1007/s11814-020-0672-5

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