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Genomic analyses of Biston suppressaria nucleopolyhedrovirus: a viral isolate obtained from the tea looper caterpillar, Biston suppressaria (Guenée, 1857)

  • Bacterial, Fungal and Virus Molecular Biology - Research Paper
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Abstract

We described the complete genome sequence of a novel baculovirus isolate of species Buzura suppressaria nucleopolyhedrovirus, called by isolate CNPSo-25. The occlusion bodies were found to be polyhedral in shape and to contain virions with singly embedded nucleocapsids. The size of the genome is 121,377 bp with a G+C content of 36.7%. We annotated 131 ORFs that cover 90.42% of the genome. Moreover, phylogenetic inference indicated that CNPSo-25 is a member of genus Alphabaculovirus that clustered together with two other Chinese isolates of the same species. We called the virus by Biston suppressaria nucleopolyhedrovirus isolate CNPSo-25 (BisuNPV-CNPSo-25), as Buzura was placed inside the lepidopteran genus Biston. As expected, we detected intra-population variability in the virus sample when the novel isolate was compared to the Chinese isolates: 292 single nucleotide variants were found in the genome, with 181 affecting the protein product. The closest representatives of other species to BisuNPV-CNPSo-25 was found to be Sucra jujuba nucleopolyhedrovirus and Hyposidra talaca nucleopolyhedrovirus, two other virus isolates of geometrid caterpillars. The study of baculovirus genomes is of importance for the development of tools for insect pest biological control and biotechnology.

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Funding

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number 428799/2018-3; 305398/2018-0 and 305468/2019-7), Coordenação de Aperfeiçoamento de Pessoal de nível Superior (CAPES/PROEX, grant number 23038.004173/2019-93), and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF, grant number 193.001532/2016).

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Authors and Affiliations

  1. Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Lucas Boeni de Oliveira & Daniel M. P. Ardisson-Araújo

  2. Embrapa Soja, Londrina, PR, 86001-970, Brazil

    Daniel Ricardo Sosa-Gómez

  3. Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, 70910-900, Brazil

    Bergmann Morais Ribeiro

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  1. Lucas Boeni de Oliveira
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  4. Daniel M. P. Ardisson-Araújo
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Correspondence to Daniel M. P. Ardisson-Araújo.

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de Oliveira, L.B., Sosa-Gómez, D.R., Ribeiro, B.M. et al. Genomic analyses of Biston suppressaria nucleopolyhedrovirus: a viral isolate obtained from the tea looper caterpillar, Biston suppressaria (Guenée, 1857). Braz J Microbiol 52, 219–227 (2021). https://doi.org/10.1007/s42770-020-00402-1

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