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MicroRNA Profiling in Mesenchymal Stromal Cells: the Tissue Source as the Missing Piece in the Puzzle of Ageing

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Abstract

Ageing is among the main risk factors for human disease onset and the identification of the hallmarks of senescence remains a challenge for the development of appropriate therapeutic target in the elderly. Here, we compare senescence-related changes in two cell populations of mesenchymal stromal cells by analysing their miRNA profiling: Human Dental Pulp Stromal Cells (hDPSCs) and human Periosteum-Derived Progenitor Cells (hPDPCs). After these cells were harvested, total RNA extraction and whole genome miRNA profiling was performed, and DIANA-miRPath analysis was applied to find the target/pathways. Only 69 microRNAs showed a significant differential expression between dental pulp and periosteum progenitor cells. Among these, 24 were up regulated, and 45 were downregulated in hDPSCs compared to hPDPCs. Our attention was centered on miRNAs (22 upregulated and 34 downregulated) involved in common pathways for cell senescence (i.e. p53, mTOR pathways), autophagy (i.e. mTOR and MAPK pathways) and cell cycle (i.e. MAPK pathway). The p53, mTOR and MAPK signaling pathways comprised 43, 37 and 112 genes targeted by all selected miRNAs, respectively. Our finding is consistent with the idea that the embryological origin influences cell behavior and the ageing process. Our study strengthens the hypothesis that ageing is driven by numerous mediators interacting through an intricate molecular network, which affects adult stem cells self-renewal capability.

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Authors and Affiliations

  1. Department of Clinical and Molecular Sciences (DISCLIMO), Università Politecnica delle Marche, 60126, Ancona, Italy

    Iolanda Iezzi, Raffaella Lazzarini, Giorgia Cerqueni, Andrell Hosein, Caterina Licini, Monia Orciani & Monica Mattioli Belmonte

  2. DISAT- Department of Applied Science and Technology, Polytechnic of Turin, Turin, Italy

    Caterina Licini

  3. Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy

    Marzia Rossato & Concetta De Quattro

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  1. Iolanda Iezzi
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Correspondence to Monica Mattioli Belmonte.

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The study was following the Marche region Ethics Board guidelines and with the 1964 Declaration of Helsinki and its later amendments. All subjects were aware that the tissue was discarded surgical material and the study did not expose them to any risk. A verbal informed consent and was obtained in lieu of a written authorization.

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Iezzi, I., Lazzarini, R., Cerqueni, G. et al. MicroRNA Profiling in Mesenchymal Stromal Cells: the Tissue Source as the Missing Piece in the Puzzle of Ageing. Stem Cell Rev and Rep 17, 1014–1026 (2021). https://doi.org/10.1007/s12015-020-10095-6

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