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Efficacy of Probiotic Milk Formula on Blood Lipid and Intestinal Function in Mild Hypercholesterolemic Volunteers: A Placebo-control, Randomized Clinical Trial

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Abstract

Several studies have reported that probiotics could modulate host lipid metabolism via altering the intestinal microbiota. Hence, the current study was aimed to assess the efficacy of a mixture of probiotic-contained milk formula (PMF) with three different bacterial strains [Lactobacillus acidophilus (La5), Lactobacillus casei (TMC), Bifidobacterium lactis (Bb12)] on lipid profile and intestinal function in healthy mild hypercholesterolemic volunteers. Totally, 40 healthy mild hypercholesterolemic subjects (180–220 mg/dL) were randomly assigned into two groups as placebo or experimental group. All the subjects were requested to drink either PMF (experimental) or skimmed milk drink formula-placebo (30 g mixed with 200 mL of water) for 10 weeks and continued by 2 weeks of the follow-up period. Supplementation of PMF for 10 weeks significantly improved (p < 0.05) the fecal weight, fecal movement (decreased fecal gastrointestinal passing time) by improving intestinal microbiota (increasing beneficial bacterial species like Lactobacillus, Bifidobacterium spp.), and lag time of low-density lipoprotein (LDL) oxidation. Also, intake of PMF substantially reduced (p < 0.05) the levels of total cholesterol (TC; 8.1%) and low-density lipoprotein cholesterol (LDL-c; 10.4%) and thus showcasing its cardioprotective efficacy. PMF considerably improves gastrointestinal function by modulating fecal movement, intestinal microbiota, and decrease cholesterol and might be helpful in the management of hypercholesterolemia.

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Acknowledgments

The author would like to thank all the participants involved in this study.

Funding

This trial was funded by Chung Shan Medical University (CSMU4212).

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Authors and Affiliations

  1. Department of Chinese Medicine, Taichung Hospital Ministry of Health and Welfare, Taichung City-40301, Taiwan, ROC

    Hui-Fang Chiu

  2. School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City-40201, Taiwan, ROC

    Chia-Yuan Fang, Kamesh Venkatakrishnan & Chin-Kun Wang

  3. School of Health Diet and Industry Management, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City-40201, Taiwan, ROC

    You-Cheng Shen

Authors
  1. Hui-Fang Chiu
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  4. Kamesh Venkatakrishnan
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  5. Chin-Kun Wang
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Contributions

Hui-Fang Chiu (HFC), Chia-Yuan Fang (CYF), and Chin-Kun Wang (CKW) involved in conceiving and designing this trial. You-Cheng Shen (YCS), Kamesh Venkatakrishnan (KV) helped in statistical analysis. CKW, KV drafted this manuscript. HFC, CYF, YCS aided in subject recruitment and conducted this trial.

Corresponding author

Correspondence to Chin-Kun Wang.

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Ethics Approval

This trial was approved by Institutional Ethical review Board Panel members of Chung Shan Medical University Hospital, Taiwan (CSMUH: CS08006) under the guidelines put forth by the “Declaration of Helsinki” and Good Clinical Practice.

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The authors declare that they have no conflict of interest.

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Chiu, HF., Fang, CY., Shen, YC. et al. Efficacy of Probiotic Milk Formula on Blood Lipid and Intestinal Function in Mild Hypercholesterolemic Volunteers: A Placebo-control, Randomized Clinical Trial. Probiotics & Antimicro. Prot. 13, 624–632 (2021). https://doi.org/10.1007/s12602-020-09728-6

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