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Pathotypes of Phytophthora sojae and their distribution in Jilin, China

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Abstract

A nine-year survey on pathotypes of Phytophthora sojae from 2007 to 2015 throughout Jilin Province of China was conducted. A total of 219 single-zoospore isolates of P. sojae that came from 250 commercial soybean fields in 25 counties were tested for their virulence phenotypes by using eight soybean differentials representing eight Rps alleles. The investigation showed that Phytophthora root rot (PRR) or P. sojae is mainly distributed in northeast Jilin. A total of 91 pathotypes were identified from 219 isolates, in which 34 matched the virulence formula of 15 known races, and two races (races 28 and 30) were identified in China for the first time. The highest isolation frequency was 6.4% found for race 8, followed by race 3 and race 1, each representing 4.6% and 4.1% of the isolates. The isolation frequencies of the remaining 12 races (races 0, 4, 9, 13, 14, 23, 24, 25, 28, 30, 33, and 44) was less than 2.3%. In addition, nine intermediate reaction types (IRTs) were found (0.5–1.4% isolation frequency). The remaining 67 pathotypes that represented approximately 66% of the isolates were not identified to a race. The isolates each defeated zero to eight Rps genes, and 71.2% of the isolates defeated more than three Rps genes, indicating that virulence of the isolates in Jilin is moderately strong. Virulence frequency of the isolates to eight single Rps genes ranged from 20–77%. More than 57% of the isolates were virulent on Rps1a, Rps1b, Rps1d, Rps6, and Rps7 while less than 30% of the isolates were virulent on Rps1c, Rps1k, and Rps3a, indicating that Rps1c, Rps1k, and Rps3a were more effective than the other five Rps genes in Jilin at reducing infection of soybean by P. sojae.

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References

  • Arsenault Labrecque G, Humira S, Amandine L, Caroline L, Genevieve M, Allen X, Francois B, Brian JK, Niklaus JG, Richard RB (2018) Stable predictive markers for Phytophthora sojae avirulence genes that impair infection of soybean uncovered by whole genome sequencing of 31 isolates. BMC Bio 16(1):80–97

    Article  Google Scholar 

  • Costamilan LM, Clebsch CC, Soares RM, Seixas DS, Godoy CV, Dorrance AE (2012) Pathogenic diversity of Phytophthora sojae pathotypes from Brazil. Eur J Plant Pathol 135:845–853

    Article  Google Scholar 

  • Cui LK, Yin WX, Tang QH, Dong SM, Zheng XB, Zhang ZG, Wang YC (2010) Distribution, pathotypes, and metalaxyl sensitivity of Phytophthora sojae from Heilongjiang and Fujian provinces in China. Plant Dis 94:881–884

    Article  Google Scholar 

  • Dai YL, Liu TF, Zhang LF, Li ZH, Guo WW, Cao S, Pan YM, Chen FX, Gao ZM (2015) First Report of Phytophthora root and stem rot caused by Phytophthora sojae on soybean in Taihe, China. Plant Dis. Doi:https://doi.org/10.1094/PDIS-04-15-0450-PDN

    Article  PubMed  Google Scholar 

  • Dorrance AE (2018) Management of Phytophthora sojae of soybean: a review and future perspectives. Can J Plant Pathol 40(2). https://doi.org/10.1080/07060661.2018.1445127

  • Dorrance AE, Schmitthenner AF (2000) New sources of resistance to Phytophthora sojae in the soybean plant introductions. Plant Dis 84:1303–1308

    Article  CAS  Google Scholar 

  • Dorrance AE, Mc Clure SA, St. Martin SK (2003) Effect of partial resistance on Phytophthora stem rot incidence and yield of soybean in Ohio. Plant Dis 87:308–312

    Article  CAS  Google Scholar 

  • Dorrance AE, Jia H, Abney TS (2004) Evaluation of soybean differentials for their interaction with Phytophthora sojae. Plant Health Progress. https://doi.org/10.1094/PHP-2004-0309-01-RS

    Article  Google Scholar 

  • Dorrance AE, Mills D, Robertson AE, Draper MA, Giesler L, Tenuta A (2007) Phytophthora root and stem rot of soybean. Plant Health Instr. https://doi.org/10.1094/PHI-I-2007-0830-07

    Article  Google Scholar 

  • Dorrance AE, Kurle JE, Robertson AE, Bradley CL (2016) Pathotype diversity of Phytophthora sojae in eleven states in the United States. Plant Dis 100(7):1429–1437

    Article  CAS  Google Scholar 

  • Gordon SG, Berry SA, St. Martin SK, Dorrance AE (2007) Genetic analysis of soybean plant introductions with resistance to Phytophthora sojae. Phytopathol. https://doi.org/10.1094/PHYTO-97-0106

    Article  Google Scholar 

  • Grijalba PE, Gally ME (2015) Virulence of Phytophthora sojae in the Pampeana subregion of Argentina from 1998 to 2004. J Phytopathol 163(9):723–730

    Article  Google Scholar 

  • Jackson TA, Kirkpatrick TL, Rupe J (2004) Races of Phytophthora sojae in Arkansas soybean fields and their effects on commonly grown soybean cultivars. Plant Dis 88(4):345–351

    Article  CAS  Google Scholar 

  • Kang IJ, Kang S, Jang IH, Jang YW, Shim HK, Heu S, Lee S (2019) Identification of new isolates of Phytophthora sojae and the reactions of Korean soybean cultivars following hypocotyl inoculation. Plant Pathol J 35(6):698–704

    Article  Google Scholar 

  • Liu CL, Yang MX, Wen JZ (2007) Molecular detection of Phytophthora sojae.. Chin J Microbiol 34:1158–1162

    CAS  Google Scholar 

  • Ma SM, Shao HT, Han XH (2013) Resistance evaluation and resistace genes analysis of soybean cultivars and new lines in Heilongjiang Province to Phytophthora root rot. Chin Agric Sci Bull 29(30):197–202

    Google Scholar 

  • Meng XQ, Shoemaker RC, Yang XB (1999) Analysis of pathogenicity and genetic variation among Phytophthora sojae isolates using RAPD. Mycol Res 103:173–178

    Article  Google Scholar 

  • Na R, Gijzen M (2016) Escaping host immunity: new tricks for plant pathogens. Plos Pathog 12(7):e1005631. https://doi.org/10.1371/journal.ppat.1005631

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schmitthenner AF (1999) Phytophthora rot of soybean. In: Hartman GL, Sinclair JB, Rupe JC (eds) Compendium of soybean diseases, 4th edn. American Phytopathological Society Press, St. Paul, pp 39–42

    Google Scholar 

  • Schmitthenner AF, Hobe M, Bhat RG (1994) Phytophthora sojae races in Ohio over a 10-year interval. Plant Dis 78:269–276

    Article  Google Scholar 

  • Shan W, Cao M, Leung D, Tyler BM (2004) The Avr1b locus of Phytophthora sojae encodes an elicitor and regulator required for avirulence on soybean plants carrying resistance gene Rps1b. Mol Plant-Microbe Interact 17:394–403

    Article  CAS  Google Scholar 

  • Shen CY, Su YC (1991) Discovery and preliminary studies of Phytophthora megasperma on soybean in China. Acta Phytopathol Sin 21:298

    Google Scholar 

  • Stewart S, Abeysekara N, Robertson AE (2014) Pathotype and genetic shifts in a population of Phytophthora sojae under soybean cultivar rotation. Plant Dis 98:614–624

    Article  CAS  Google Scholar 

  • Sui Z, Huang J, Ma Z, Shi X, Xie J, Wen J (2010) Evaluation of resistant genes to phytophthora root and stem rot in soybean from Jilin and Liaoning provinces. Chin J Oil Crop Sci 32(1):094–098

    Google Scholar 

  • Tian M, Zhao LM, Li S, Huang J, Sui Z, Wen JZ (2016) Pathotypes and metalaxyl sensitivity of Phytophthora sojae and their distribution in Heilongjiang, China 2011–2015. J Gen Plant Pathol 82:132–141

    Article  CAS  Google Scholar 

  • Wang ZY (2007) Identification and isolation of soybean root rot in Lingbi, Anhui. Chin J Anhui Inst Educ 25:96–98

    Google Scholar 

  • Wang H, Gao F, Wu CX, Zhan Y, Zhang J, Li GY (2008) Identification on biological traits and physiological race of Phytophthora sojae in Xinjiang. Chin J Xinjiang Agric Sci 45:126–128

    Google Scholar 

  • Wen JZ, Chen HY (2002) Races and virulence phenotypes of Phytophthora sojae in Heilongjiang province and Mongolia Region of China. Chin J Oil Crop Sci 24:63–66

    Google Scholar 

  • Wen JZ, Zhang MH (1998) The pathogen causing Phytophthora disease of soybean. Chin J Oil Crop Sci 20:76–78

    Google Scholar 

  • Wen JZ, Jie Y, Tian M, Chen YF, Zhang B, Wu WX, Wu D, Zhao YQ, Gao XY (2017) Spatial-temporal dynamics of Phytophthora sojae on population structure in the east of Heilongjiang Province. Chin J Northeast Agric Univ 45(8):1–8

    Google Scholar 

  • Xu XH, Lv HY, Qu JJ, Yang QK (2003) Phytophthora sojae races in northeast of China and the virulence evaluation of the isolates. Chin J Acta Phytophylacica Sin 30:125–128

    Google Scholar 

  • Xue AG, Marchand M, Chen YH, Zhang SZ, Cober ER, Tenuta A (2015) Races of Phytophthora sojae in Ontario, Canada, 2010–2012. Can J Plant Pathol 37:376–383

    Article  Google Scholar 

  • Zhang LF (2010) Isolation, identification and pathotypes of Phytophthora sojae from Anhui province. Doctor degree dissertation of Anhui Agricultural University, China

  • Zhang JQ, Sun SL, Wang GQ, Duan CX, Wang XM, Wu XF, Zhu ZD (2014) Characterization of Phytophthora resistance in soybean cultivars/lines bred in Henan province. Euphytica 196:375–384

    Article  Google Scholar 

  • Zhu ZD, Wang XM, Chang RZ, Ma SM, Wu XF, Tian YL (2000) Identification of race of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang province. Chin J Sci Agric Sin 33:62–67

    Google Scholar 

  • Zhu ZD, Wang XM, Wang HB, Wu XF, Zhang L (2001) Identification and race of Phytophthora sojae isolates collected in Mengcheng, Anhui province. Chin J Acta Phytopathol Sin 31:236–240

    Google Scholar 

Download references

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 31670444, 31370449 and 32071637) to Wen Jingzhi.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Zhuoqun Zhang, Yuqi Zhao, Han Yu, Yufei Chen & Jingzhi Wen

  2. Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi, 154007, People’s Republic of China

    Xin Gu

Authors
  1. Zhuoqun Zhang
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  2. Yuqi Zhao
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  3. Han Yu
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  4. Yufei Chen
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  5. Xin Gu
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  6. Jingzhi Wen
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Zhang Zhuoqun], [Zhao Yuqi], [Yu Han], [Chen Yufei] and [Gu Xin]. The first draft of the manuscript was written by [Zhang Zhuoqun] with the help of [Wen Jingzhi] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jingzhi Wen.

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Zhang, Z., Zhao, Y., Yu, H. et al. Pathotypes of Phytophthora sojae and their distribution in Jilin, China. J Plant Pathol 103, 241–248 (2021). https://doi.org/10.1007/s42161-020-00723-y

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