Abstract
In soybean, only one mitochondrial genome of cultispecies has been completely obtained. To explore the effect of mitochondrial genome on soybean cytoplasmic male sterility (CMS), two CMS lines and three maintainer lines were used for sequencing. Comparative analysis showed that mitochondrial genome of the CMS line was more compact than that of its maintainer line, but genes were highly conserved. Conserved and unique sequence coexisted in the genomes. Mitochondrial genomes contained different sequence lengths and copy numbers of repeats between CMS line and maintainer line. Large and short repeats mediated intramolecular and intermolecular recombination in mitochondria. Unique sequences and genes were also involved in recombination process and constituted a complex network. orf178 and orf261 were identified as CMS-associated candidate genes. They had sequence characteristics of reported CMS genes in other crops and could be transcribed in CMS lines but not in maintainer lines. This report reveals mitochondrial genome of soybean CMS lines and compares complete mitochondrial sequence between CMS lines and their maintainer lines. The information will be helpful in further understanding the characteristics of soybean mitochondrial genome and the mechanism underlying CMS.
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Funding
Our work was supported by the National Key R & D Program of China (2016YFD0101500, 2016YFD0101504); the Fundamental Research Funds for the Central Universities (KYT201801); and the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55).
Authors’ contributionsSY and TH proposed the research plans and designed experiments; TH, XD, HZ, YL, LC, TW, LY and ZN jointly performed the experiments; TH analyzed the data; TH wrote the manuscript; and SY, QS and JG revised the manuscript. All authors read and approved the final manuscript.
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Mitochondrial sequences are deposited at the GenBank data library with accession numbers SRR13077102 (NJCMS1A), SRR13077101 (NJCMS1B-1), SRR13077100 (NJCMS1B-2), SRR13077099 (NJCMS1B-3), SRR13077098 (NJCMS4A), SRR13077097 (NJCMS4B) and SRR13077096 (NJCMS5B).
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He, T., Ding, X., Zhang, H. et al. Comparative analysis of mitochondrial genomes of soybean cytoplasmic male-sterile lines and their maintainer lines. Funct Integr Genomics 21, 43–57 (2021). https://doi.org/10.1007/s10142-020-00760-x
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DOI: https://doi.org/10.1007/s10142-020-00760-x