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Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells

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Abstract

Our ultimate aim is to establish a small-bodied surrogate broodstock (e.g., mackerel) that produces functional gametes of the Pacific bluefin tuna Thunnus orientalis using xenogeneic germ cell transplantation. To accomplish this goal, we need to be able to sequentially trace transplanted donor cells in recipients. Here we succeeded in identifying two monoclonal antibodies that could specifically recognize the germ cells of bluefin tuna but not of the chub mackerel Scomber japonicus or the eastern little tuna Euthynnus affinis, which are promising recipient species. Using these antibodies, we developed a method to detect transplanted bluefin tuna germ cells in the gonads of xenogenic recipients. Since all stages of testicular germ cells can be recognized by these antibodies, donor germ cells can be observed throughout gametogenesis in the recipient’s gonads. Thus, the antibodies established in this study could be a powerful tool to observe survival, proliferation, and differentiation of the transplanted bluefin tuna germ cells in the gonads of small Scombridae recipients, and evaluate transplantation efficiency.

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Acknowledgements

This work was supported by MEXT “sophistication of technologies to secure living marine resources” Grant Number JPMXD0511102331.

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Authors and Affiliations

  1. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan Minato-ku, Tokyo, 108-8477, Japan

    Ryosuke Yazawa, Tsubasa Kubokawa, Wataru Kawamura, Reoto Tani, Shigeharu Kamio & Goro Yoshizaki

  2. Institute for Reproductive Biotechnology for Aquatic Species, Tokyo University of Marine Science and Technology, 4-5-7 Konan Minato-ku, Tokyo, 108-8477, Japan

    Ryosuke Yazawa, Kensuke Ichida, Tetsuro Morita & Goro Yoshizaki

Authors
  1. Ryosuke Yazawa
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  2. Tsubasa Kubokawa
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  3. Kensuke Ichida
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  4. Wataru Kawamura
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  5. Reoto Tani
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  6. Shigeharu Kamio
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  7. Tetsuro Morita
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  8. Goro Yoshizaki
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Correspondence to Ryosuke Yazawa.

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Yazawa, R., Kubokawa, T., Ichida, K. et al. Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells. Fish Sci 87, 105–112 (2021). https://doi.org/10.1007/s12562-020-01486-2

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  • DOI: https://doi.org/10.1007/s12562-020-01486-2

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