Abstract
Cultivation of high-lipid microalgae using municipal sewage can both treat polluted water and gain biodiesel. Rapid cultivation of microalgae is crucial for the entire process. In this study, four stimulants (phytohormone 2,4-d; Fe3+; photosynthetic bacteria—Rhodopseudomonas palustris; CO2) were firstly chosen to investigate their effects on microalgae Chlorella pyrenoidosa cultivation in municipal sewage. Meanwhile, orthogonal experiments were designed to compare the enhancing effects on the growth of microalgae C. pyrenoidosa and lipid accumulation by different combinations of stimulants, which were also conducted for outdoor culture. The optimum stimulant combination was 1.0 mg/L 2,4-d; 10−7 mol/L Fe3+; 4.5 mL (109 cells/mL) photosynthetic bacteria; and 8% CO2. Among the four kinds of stimulants, phytohormone 2,4-d and photosynthetic bacteria played more important roles on the microalgae growth and lipid accumulation. The optimum combination of stimulants could also promote the microalgae biomass and lipid yield during outdoor cultivation. The maximum OD681 of microalgae was 37.9% higher than that of the control group without addition of stimulants. The obtained microalgae dry weight and lipid production were 0.3 and 0.13 g /L, which were 80.0% and 85.7% higher than that of the control group, respectively. The promotion of microalgae photosynthetic rate was the main reason for the stimulant-enhanced microalgae cultivation. This study exerts positive effects on the development of microalgae biodiesel.
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References
Supaporn P, Yeom SH (2017) Optimization of a one-step direct process for biodiesel production from blended sewage sludge. Korean J Chem Eng 34(2):360–365
Wang H, Zhang W, Chen L, Wang J, Liu T (2013) The contamination and control of biological pollutants in mass cultivation of microalgae. Bioresour Technol 128:745–750
Cheng J, Lu H, Huang Y, Li K, Huang R, Zhou J, Cen K (2016) Enhancing growth rate and lipid yield of Chlorella with nuclear irradiation under high salt and CO2 stress. Bioresour Technol 203:220–227
Liu Z, Wang G, Zhou B (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour Technol 99(11):4717–4722
Ulloa G, Otero A, Sánchez M, Sineiro J, Núñez MJ, Fábregas J (2012) Effect of Mg, Si, and Sr on growth and antioxidant activity of the marine microalga Tetraselmis suecica. J Appl Phycol 24(5):1229–1236
François MMM, Kustka AB, Shaked Y (2008) The role of unchelated Fe in the iron nutrition of phytoplankton. Limnol Oceanogr 53(1):400–404
Tate JJ, Gutierrez-Wing MT, Rusch KA, Benton MG (2013) The effects of plant growth substances and mixed cultures on growth and metabolite production of green algae Chlorella sp.: a review. J Plant Growth Regul 32(2):417–428
Stirk WA, van Staden J (2020) Potential of phytohormones as a strategy to improve microalgae productivity for biotechnological applications. Biotechnol Adv 44:107612
Dao G, Wu G, Wang X, Zhuang L, Zhang T, Hu H (2018) Enhanced growth and fatty acid accumulation of microalgae Scenedesmus sp LX1 by two types of auxin. Bioresour Technol 247:561–567
De-Bashan LE, Hernandez J, Morey T, Bashan Y (2004) Microalgae growth-promoting bacteria as “helpers” for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater. Water Res 38(2):466–474
Ogar A, Sobczyk L, Turnau K (2015) Effect of combined microbes on plant tolerance to Zn-Pb contaminations. Environ Sci Pollut R 22(23):19142–19156
Ying C, Chang M, Chang Y, Chao W, Yeh S, Hsu J (2020) Photosynthetic bacteria enhanced water quality and integrity of microbial community composition of integrated multitrophic aquaculture system of milkfish Chanos chanos coastal farming. Fish Sci 86(2):329–338
Kumar PK, Krishna SV, Naidu SS, Verma K, Bhagawan D, Himabindu V (2019) Biomass production from microalgae Chlorella grown in sewage, kitchen wastewater using industrial CO2 emissions: comparative study. Carbon Resources Conversion 2(2):126–133
Rahaman MSA, Cheng L, Xu X, Zhang L, Chen H (2011) A review of carbon dioxide capture and utilization by membrane integrated microalgal cultivation processes. Renew Sust Energ Rev 15(8):4002–4012
Tu R, Jin W, Wang M, Han S, Abomohra AE, Wu W (2016) Improving of lipid productivity of the biodiesel promising green microalga Chlorella pyrenoidosa via low-energy ion implantation. J Appl Phycol 28(4):2159–2166
Qu F, Jin W, Zhou X, Wang M, Chen C, Tu R, Han SF, He Z, Li SF (2020) Nitrogen ion beam implantation for enhanced lipid accumulation of Scenedesmus obliquus in municipal wastewater. Biomass Bioenergy 134:105483
Zhou X, Jin W, Wang Q, Guo S, Tu R, Han S, Chen C, Xie G, Qu F, Wang Q (2020) Enhancement of productivity of Chlorella pyrenoidosa lipids for biodiesel using co-culture with ammonia-oxidizing bacteria in municipal wastewater. Renew Energy 151:598–603
APHA (2012) Standard methods for the evaluation of water and wastewater, 22nd edn. American Public Health Association, Washington DC
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917
Wang Q, Jin W, Zhou X, Guo S, Gao S, Chen C et al (2019) Growth enhancement of biodiesel-promising microalga Chlorella pyrenoidosa in municipal wastewater by polyphosphate-accumulating organisms. J Clean Prod 240:118148
Bradley PM (1991) Plant hormones do have a role in controlling growth and development of algae. J Phycol 27(3):317–321
Stuetz RM, Greene AC, Madgwick JC (1996) Microalgal-facilitated bacterial oxidation of manganese. J Ind Microbiol 16(5):267–273
GB18918-2002, (2002) Discharge standard of pollutants for municipal wastewater treatment plant. Chinese Environmental Protection Administration, Beijing
Lv X, Hu H, Zhang X, Cong W, Tan T (2005) Effects of Fe~(3+) on growth and photosynthesis of Oscillatoria planctonica. Acta Hydrobiol Sin 29:318–322
Rana MS, Bhushan S, Sudhakar DR, Prajapati SK (2020) Effect of iron oxide nanoparticles on growth and biofuel potential of Chlorella spp. Algal Res 49(101942):101942
Alderkamp A, van Dijken GL, Lowry KE, Connelly TL, Lagerström M, Sherrell RM et al (2015) Fe availability drives phytoplankton photosynthesis rates during spring bloom in the Amundsen Sea Polynya, Antarctica. Elementa: Science of the Anthropocene 3(000043):43
Mohsin T, Naqvi FN (2007) Auxin-mediated regulation of early growth activities in Vigna radiata: induction and repression of two hydrolases, invertase and α-amylase. J Food Agric Environ 5(2):125–127
Yu Z, Song M, Pei H, Jiang L, Hou Q, Nie C, Zhang L (2017) The effects of combined agricultural phytohormones on the growth, carbon partitioning and cell morphology of two screened algae. Bioresour Technol 239:87–96
Wu G (2004) The action mechanism of auxin. Research on Agricultural Mechanization 3(06):33–37
Zhou Q, Zhang P, Zhang G (2014) Biomass and carotenoid production in photosynthetic bacteria wastewater treatment: effects of light intensity. Bioresour Technol 171:330–335
Najafpour G, Ismail KSK, Younesi H, Mohamed AR, Kamaruddin AH (2004) Hydrogen as clean fuel via continuous fermentation by anaerobic photosynthetic bacteria, Rhodospirillum rubrum. Afr J Biotechnol 3(10):4
Ludden PW, Roberts GP (2002) Nitrogen fixation by photosynthetic bacteria. Photosynth Res 73(1-3):115–118
Gao Y, Yang S, Li S, Zhang Q, Tian W, Zheng J et al (2014) Effects of photosynthetic bacteria on growth and photosynthetic function in wheat. J Microbiol 41(06):1152–1159
Dahms H, Ying X, Pfeiffer C (2006) Antifouling potential of cyanobacteria: a mini-review. Biofouling 22(5):317–327
Shen Q, Jiang J, Chen L, Cheng L, Xu X, Chen H (2015) Effect of carbon source on biomass growth and nutrients removal of Scenedesmus obliquus for wastewater advanced treatment and lipid production. Bioresour Technol 190:257–263
Voltolina D, Cordero B, Nieves M, Soto LP (1999) Growth of Scenedesmus sp. In artificial wastewater. Bioresour Technol 68(3):265–268
Woertz I, Feffer A, Lundquist T, Nelson Y (2009) Algae grown on dairy and municipal wastewater for simultaneous nutrient removal and lipid production for biofuel feedstock. J Environ Eng-ASCE 135(11):1115–1122
Ryu B, Kim EJ, Kim H, Kim J, Choi Y, Yang J (2014) Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria. Biotechnol Bioproc E 19(2):201–210
Han S, Jin W, Tu R, Abomohra AE, Wang Z (2016) Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater. Bioprocess Biosyst Eng 39(7):1073–1079
Reddy HK, Muppaneni T, Sun Y, Li Y, Ponnusamy S, Patil PD et al (2014) Subcritical water extraction of lipids from wet algae for biodiesel production. Fuel 133:73–81
Nematian T, Salehi Z, Shakeri A (2020) Conversion of bio-oil extracted from Chlorella vulgaris micro algae to biodiesel via modified superparamagnetic nano-biocatalyst. Renew Energy 146:1796–1804
Zhang X, Yan S, Tyagi RD, Surampalli RY (2013) Energy balance and greenhouse gas emissions of biodiesel production from oil derived from wastewater and wastewater sludge. Renew Energy 55:392–403
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25(3):294–306
Park W, Yoo G, Moon M, Kim CW, Choi Y, Yang J (2013) Phytohormone supplementation significantly increases growth of chlamydomonas reinhardtii cultivated for biodiesel production. Appl Biochem Biotechnol 171(5):1128–1142
Funding
This work was supported by the National Natural Science Foundation of China (No. 51878215); Natural Science Foundation of Guangdong Province, China (2018A030313185); Key Areas Research and Development Program of Guangdong Province, China (2019B110205001); Demonstration Project for Marine Economic Development in Shenzhen to Dr. Zhangli HU, China’s State Oceanic Administration; and Shenzhen Science and Technology Innovation Project (KJYY20171011144235970).
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Wang, Q., Jin, W., Zhou, X. et al. Enhancement of biodiesel-promising microalgae Chlorella pyrenoidosa growth using stimulants in municipal sewage. Biomass Conv. Bioref. 13, 813–820 (2023). https://doi.org/10.1007/s13399-020-01204-z
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DOI: https://doi.org/10.1007/s13399-020-01204-z