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Productivity of Pseudomonas putida TISTR 1522 in polyhydroxyalkanoates (PHAs) production from saponified palm oil

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Abstract

Polyhydroxyalkanoates (PHAs) have attracted attention as an environmentally degradable bioplastic which potentially replaces synthetic polymers used in a wide range of industries. One of most promising microorganisms for the production of PHAs is Pseudomonas putida. In this study, we purpose to develop sustainable processes to convert abundant palm oil available in local market to high value PHAs and optimize PHAs production by Pseudomonas putida TISTR 1522 from saponified palm oil. We found that the highest yield of PHAs production (0.95 g/L, 40.15%) was obtained in culture medium supplemented with 1% (w/v) fatty acid salt by P. putida TISTR 1522 after 24-h cultivation. The intracellular PHAs were located in granules inside the cells, which fluoresced bright yellow by staining with Nile red. The physical appearance of intracellular PHAs investigated by transmission electron microscope (TEM) revealed that PHAs accumulate in granules, about 3–10 granules per cell. These granules are white and roundish-shaped with 0.3–0.5-μm diameter. The 1H NMR spectrum represented the typical characters of medium-chain length-PHAs. This variation of all parameters was successfully demonstrated a good intracellular PHAs accumulation in P. putida TISTR 1522 by fatty acid salt utilization.

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All data generated or analyzed during this study are included in this published article.

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Funding

The authors are thankful to the financial support from King Mongkut’s University of Technology North Bangkok (KMUTNB-Post-63-06, KMUTNB-62-KNOW-08 and KMUTNB-63-KNOW-17), Royal Academy of Engineering (RAEng, UK) and Office of Higher Education Commission (OHEC, Thailand)-Industry Academy Partnership Program (IAPP18-19\75).

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Conceptualization: all named authors; Methodology: all named authors; Formal analysis and investigation: all named authors; Writing—original draft preparation: Pornpanna Thenchartanan; Writing—review and editing: Nipon Pisutpaisal; Funding acquisition: all named authors; Resources: all named authors; Supervision: all named authors. We confirm that the manuscript has been read and approved by all named authors. We confirm that the order of authors listed in the manuscript has been approved by all named authors.

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Correspondence to Nipon Pisutpaisal.

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Boonyawanich, S., Tanikkul, P., Thenchartanan, P. et al. Productivity of Pseudomonas putida TISTR 1522 in polyhydroxyalkanoates (PHAs) production from saponified palm oil. Appl Biochem Biotechnol 193, 1086–1098 (2021). https://doi.org/10.1007/s12010-020-03481-w

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