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In vitro inhibitory effect of maraviroc on the association of the simian immunodeficiency virus envelope glycoprotein with CCR5

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Abstract

Asian macaques infected with simian immunodeficiency viruses (SIVs) isolated from African non-human primates develop a disease similar to human AIDS. SIV enters its target cells by binding to CD4 and a coreceptor, typically CCR5. Maraviroc is an entry inhibitor of human immunodeficiency virus type 1 (HIV-1) that prevents the interaction between CCR5 and the surface subunit gp120 of the viral envelope glycoprotein (Env). Thus far, the activity of maraviroc on SIV entry has been poorly studied. Here, we determined in vitro pharmacological parameters of the effect of maraviroc on the SIV Env association with CCR5. Cell-to-cell fusion inhibition assays were used to compare the susceptibility to maraviroc of the SIVsmmPBj Env-CCR5 interaction with that of HIV-1BaL Env. Analysis of dose–response curves and determination of IC50 values demonstrate that increasing concentrations of maraviroc inhibit the membrane fusion activity of SIVsmmPBj Env in a manner and to an extent similar to that of HIV-1BaL Env.

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Funding

This research was funded by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina).

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Authors and Affiliations

  1. Laboratorio de Virología, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad de Belgrano, Villanueva 1324, C1426BMJ, Buenos Aires, Argentina

    Ignacio Giraudy, César A. Ovejero, José L. Affranchino & Silvia A. González

Authors
  1. Ignacio Giraudy
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  2. César A. Ovejero
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  3. José L. Affranchino
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  4. Silvia A. González
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Contributions

Conceptualization, JLA and SAG; methodology, JLA and SAG; validation, IG, CAO, JLA and SAG; formal analysis, IG, CAO, JLA and SAG; investigation, IG, CAO, JLA and SAG; resources, JLA and SAG; data curation, JLA and SAG; writing-original draft preparation, JLA and SAG; writing-review and editing, IG, CAO, JLA and SAG; visualization, JLA and SAG; supervision, JLA and SAG; project administration, JLA and SAG; funding acquisition, JLA and SAG.

Corresponding author

Correspondence to Silvia A. González.

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The authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Juergen A. Richt.

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Giraudy, I., Ovejero, C.A., Affranchino, J.L. et al. In vitro inhibitory effect of maraviroc on the association of the simian immunodeficiency virus envelope glycoprotein with CCR5. Virus Genes 57, 106–110 (2021). https://doi.org/10.1007/s11262-020-01816-7

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  • DOI: https://doi.org/10.1007/s11262-020-01816-7

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