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Effect of divalent manganese (Mn2+) concentration on the growth and nitrate nitrogen content of lettuce during aeroponic intercropping with cherry radish

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Abstract

Manganese (Mn) plays an important role in regulating metabolism, especially nitrogen metabolism, in plants. Considering the desired levels required for plant growth and the most popular concentration in nutrient solutions in soilless cultures, lettuce plants were exposed to 4 µM, 10 µM, or 40 µM Mn2+ as MnSO4·4H2O during aeroponic intercropping with cherry radish plants with a 1:1 quantity ratio of lettuce/cherry radish. The effects of Mn2+ on plant growth, nitrate nitrogen (NO3−-N), and metabolism of lettuce were investigated. The results showed that the fresh weight (FW) and dry weight (DW) of lettuce increased by 20.9% and 24.7%, respectively, at 30 days after transplanting when the Mn2+ concentration ranged from 4 (treatment C1) to 40 µM (treatment C3). The NO3−-N content in the edible parts of lettuce decreased by 34.4% and 44.9% with increasing Mn2+ concentrations on the 10th day and the 20th day after transplanting, respectively, but the maximal reduction of the NO3−-N content was only 9% on the 30th day when the Mn2+ concentration ranged from 4  (treatment C1) to 40 µM (treatment C3). Additionally, our results showed that increased but not excess Mn2+ could markedly promote nitrate reductase (NR) activity instead of limiting the stomata, which was one reason why the NO3−-N content in edible parts decreased. During aeroponic intercropping with cherry radish plants, Mn2+ thresholds were found that improved organic biomass and nitrogen assimilation in the edible parts of lettuce. The Mn2+ thresholds could be similar or different, but both were within the range of 10  (treatment C2) –40 µM (treatment C3).

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Dr. Wei Cai for his helpful advice on language and assays. The financial support provided by Jilin Scientific and Technological Development Program (20170204020N Y) for this research is greatly appreciated.

Funding

This study was funded by Jilin Scientific and Technological Development Program (grant number 20170204020N Y).

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Authors and Affiliations

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun, China

    Lei Zhang, Linlin Wang, Faqinwei Li, Fei Xiao & Haiye Yu

  2. School of Science, Changchun Institute of Technology, Jilin University, Changchun, China

    Linlin Wang

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  1. Lei Zhang
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Zhang, L., Wang, L., Li, F. et al. Effect of divalent manganese (Mn2+) concentration on the growth and nitrate nitrogen content of lettuce during aeroponic intercropping with cherry radish. Hortic. Environ. Biotechnol. 62, 243–251 (2021). https://doi.org/10.1007/s13580-020-00303-0

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