Abstract
Bioaerosols are airborne microorganisms that cause infectious sickness, respiratory and chronic health issues. They have become a latent threat, particularly in indoor environment. Photocatalysis is a promising process to inactivate completely bioaerosols from air. However, in systems treating a continuous air flow, catalysts can be partially lost in the gaseous effluent. To avoid such phenomenon, supporting materials can be used to fix catalysts. In the present work, four photocatalytic systems using Perlite or Poraver glass beads impregnated with ZnO or TiO2 were tested. The inactivation mechanism of bioaerosols and the cytotoxic effect of the catalysts to bioaerosols were studied. The plug flow photocatalytic reactor treated a bioaerosol flow of 460 × 1 06 cells/m3air with a residence time of 5.7 s. Flow Cytometry (FC) was used to quantify and characterize bioaerosols in terms of dead, injured and live cells. The most efficient system was ZnO/Perlite with 72% inactivation of bioaerosols, maintaining such inactivation during 7.5 h due to the higher water retention capacity of Perlite (2.8 mL/gPerlite) in comparison with Poraver (1.5 mL/gPerlite). However, a global balance showed that TiO2/Poraver system triggered the highest level of cytotoxicity to bioaerosols retained on the support after 96 h with 95% of dead cells. SEM and FC analyses showed that the mechanism of inactivation with ZnO was based on membrane damage, morphological cell changes and cell lysis; whereas only membrane damage and cell lysis were involved with TiO2. Overall, results highlighted that photocatalytic technologies can completely inactivate bioaerosols in indoor environments.
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Álvarez-Hornos J, Gabaldón C, Martínez-Soria V, Marzal P, Penya-roja J M, Sempere F (2007). Biofiltration of ethyl acetate under continuos and intermittent loading. Environmental Progress & Sustainable Energy, 26(4): 327–337
Anicua R, de Carmen G M (2009). Particule size and micromorphological relation on physical properties of perlite and zeolite. Agricultura Técnica en México, 35(2): 147–156
Baysal A, Saygin H, Ustabasi G S (2018). Interaction of PM2.5 airborne particulates with ZnO and TiO2 nanoparticles and their effect on bacteria. Environmental Monitoring and Assessment, 190(1): 34–49
Boyjoo Y, Sun H, Liu J, Pareek V K, Wang S (2017). A review on photocatalysis for air treatment: From catalyst development to reactor design. Chemical Engineering Journal, 310(2): 537–559
Cárdenas C, Tobón J I, García C, Vila J (2012). Functionalized building materials: Photocatalytic abatement of NOx by cement pastes blended with TiO2 nanoparticles. Construction & Building Materials, 36: 820–825
Cendrowski K, Peruzynska M, Markowska-Szczupak A, Chen X, Wajda A, Lapczuk J, Kurzawski M, Kalenczuk R J, Drozdzik M, Mijowska E (2013). Mesoporous silica nanospheres functionalized by TiO2 as a photoactive antibacterial agent. Journal of Nanomedicine & Nanotechnology, 4(6): 1–6
Chuaybamroong P, Chotigawin R, Supothina S, Sribenjalux P, Larpkiattaworn S, Wu C Y (2010). Efficacy of photocatalytic HEPA filter on microorganism removal. Indoor Air, 20(3): 246–254
Esquivel-Gonzalez S, Aizpuru A, Patrón-Soberano A, Arriaga S (2017). Characterization of bioaerosol emissions from two biofilters during treatment of toluene vapours using epifluorescence microscopy. International Biodeterioration & Biodegradation, 123: 78–86
García-Pérez T, Aizpuru A, Arriaga S (2013). By-passing acidification limitations during the biofiltration of high formaldehyde loads via the application of ozone pulses. Journal of Hazardous Materials, 262: 732–740
Hinojosa-Reyes M, Arriaga S, Diaz-Torres L A, Rodríguez-González V (2013). Gas-phase photocatalytic decomposition of ethylbenzene over perlite granules coated with indium doped TiO2. Chemical Engineering Journal, 224: 106–113
Hosseini S N, Borghei S M, Vossoughi M, Taghavinia N (2007). Immobilization of TiO2 on perlite granules for photocatalytic degradation of phenol. Applied Catalysis B: Environmental, 74(1–2): 53–62
Humbal C, Gautam S, Trivedi U (2018). A review on recent progress in observations, and health effects of bioaerosols. Environment International, 118: 189–193
Kumar R, Umar A, Kumar G, Nalwa H S (2017). Antimicrobial properties of ZnO nanomaterials: A review. Ceramics International, 43(5): 3940–3961
Lee K M, Lai C W, Ngai K S, Juan J C (2016). Recent developments of zinc oxide based photocatalyst in water treatment technology: A review. Water Research, 88: 428–448
Li Y, Zhang W, Niu J, Chen Y (2012). Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. ACS Nano, 6(6): 5164–5173
Lingampalli S R, Ayyub M M, Rao C N R (2017). Recent progress in the photocatalytic reduction ofcarbon dioxide. ACS Omega, 2(6): 2740–2748
Muñoz R, Arriaga S, Hernández S, Guieysse B, Revah S (2006). Enhanced hexane biodegradation in a two phase partitioning bioreactor: Overcoming pollutant transport limitations. Process Biochemistry, 41(7): 1614–1619
Niazi S, Hassanvand M S, Mahvi A H, Nabizadeh R, Alimohammadi M, Nabavi S, Faridi S, Dehghani A, Hoseini M, Moradi-Joo M, Mokamel A, Kashani H, Yarali N, Yunesian M (2015). Assessment of bioaerosol contamination (bacteria and fungi) in the largest urban wastewater treatment plant in the Middle East. Environmental Science and Pollution Research International, 22(20): 16014–16021
Ong C B, Ng L Y, Mohammad A W (2018). A review of ZnO nanoparticles as solar photocatalysts: Synthesis, mechanisms and applications. Renewable & Sustainable Energy Reviews, 81: 536–551
Pagalilauan H A M, Paraoan C E M, Vital P G (2018). Detection of pathogenic bioaerosols and occupational risk in a Philippine landfill site. Archives of Environmental & Occupational Health, 73(2): 107–114
Pinho L, Mosquera M J (2013). Photocatalytic activity of TiO2-SiO2 nanocomposites applied to buildings: Influence of particle size and loading. Applied Catalysis B: Environmental, 134–135: 205–221
Rodrigues-Silva C, Miranda S M, Lopes F V S, Silva M, Dezotti M, Silva A M T, Faria J L, Boaventura R A R, Vilar V J P, Pinto E (2017). Bacteria and fungi inactivation by photocatalysis under UVA irradiation: liquid and gas phase. Environmental Science and Pollution Research International, 24(7): 6372–6381
Sánchez B, Sánchez-Muñoz M, Muñoz-Vicente M, Cobas G, Portela R, Suárez S, Gonzalez A E, Rodriguez N, Amils R (2012). Photocatalytic elimination of indoor air biological and chemical pollution in realistic conditions. Chemosphere, 87(6): 625–630
Saucedo-Lucero J O, Arriaga S (2013). Photocatalytic degradation of hexane vapors in batch and continuous systems using impregnated ZnO nanoparticles. Chemical Engineering Journal, 218: 358–367
Saucedo-Lucero J O, Quijano G, Arriaga S, Muñoz R (2014). Hexane abatement and spore emission control in a fungal biofilter-photoreactor hybrid unit. Journal of Hazardous Materials, 276: 287–294
Sing K S W, Everett D H, Haul R A W, Moscou L, Pierotti R S, Rouquerol J, Siemieniewsky T (1985). Reporting physisorption data for gas/solid systems with special reference to the determination of surface area and porosity. Pure and Applied Chemistry, 57(4): 603–619
Valdez-Castillo M, Saucedo-Lucero J O, Arriaga S (2019). Photocatalytic inactivation of airborne microorganisms in continuous flow using perlite-supported ZnO and TiO2. Chemical Engineering Journal, 374: 914–923
Wang C, Xi J Y, Hu H Y (2009). Reduction of toxic products and bioaerosol emission of a combined ultraviolet-biofilter process for chlorobenzene treatment. Journal of the Air & Waste Management Association, 59(4): 405–410
WHO (2020). WHO Characterizes Coronavirus Disease (COVID-19) as a pandemic. Geneva: World Health Organization
Wu B, Wang Y, Lee Y H, Horst A, Wang Z, Chen D R, Sureshkumar R, Tang Y J. (2010). Comparative eco-toxicities of nano-ZnO particles under aquatic and aerosol exposure modes. Environmental Science & Technology, 44(4): 1484–1489
Wu F, Zhao S, Yu B, Chen Y M, Wang W, Song Z G, Hu Y, Tao Z, Tian J, Pei Y, Yuan M, Zhang Y, Dai F, Liu Y, Wang Q, Zheng J, Xu L, Holmes E C, Zhang Y (2020). A new coronavirus associated with human respiratory disease in China. Nature, 579(7798): 265–269
Xie Y, He Y, Irwin P L, Jin T, Shi X (2011). Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Applied and Environmental Microbiology, 77(7): 2325–2331
Zhong L, Haghighat F (2015). Photocatalytic air cleaners and materials technologies: Abilities and limitations. Building and Environment, 91: 191–203
Acknowledgements
This work was financially supported by CONACYT from the project CB-2014-01-239622. M.V.C was supported by a National CONACYT scholarship. We thank M.Sc. Ana Iris Peña Maldonado, Karla Lizeth Villalobos-Romero, Dr. Olga Araceli Patrón-Soberano and Dr. Guadalupe Gutierrez Escobedo, M.Sc. Carmen Rocha Medina, Guadalupe Ortega Salazar for technical support. We are also grateful for the use of infrastructure of the National laboratories LINAN. Special gratitude to Dr. Aitor Aizpuru to improve the manuscript.
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Highlights
• ZnO/Perlite inactivated 72% of bioaerosols in continuous gas phase.
• TiO2 triggered the highest level of cytotoxicity with 95 % dead cells onto Poraver.
• Inactivation mechanism occurred by membrane damage, morphological changes and lysis.
• ZnO/Poraver showed null inactivation of bioaerosols.
• Catalysts losses at the outlet of the photoreactor for all systems were negligible.
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Response of bioaerosol cells to photocatalytic inactivation with ZnO and TiO2 impregnated onto Perlite and Poraver carriers
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Valdez-Castillo, M., Arriaga, S. Response of bioaerosol cells to photocatalytic inactivation with ZnO and TiO2 impregnated onto Perlite and Poraver carriers. Front. Environ. Sci. Eng. 15, 43 (2021). https://doi.org/10.1007/s11783-020-1335-9
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DOI: https://doi.org/10.1007/s11783-020-1335-9