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Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo

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Abstract

The cleavage pattern of a vertebrate’s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde (PUA), 2,4-decadienal (DD)—a model aldehyde for experimental studies—adversely affects the developing embryos of several aquatic species. In this study, we employed DD for inhibition of cleavage of a definite blastomere in sturgeon embryos under various conditions. The effective treatment was found to be a combination of DD injection (0.01 v/v) and visible light (44.86–91.15 W m−2). Notably, DD injection or light irradiation alone cannot inhibit cleavage. Furthermore, spatial RNA localization analysis using quantitative polymerase chain reaction (qPCR)-tomography revealed that the localized pattern of selected maternal messenger ribonucleic acids (mRNAs) remained constant along the animal–vegetal (A-V) axis, which suggests that RNA localization is completed by the end of oogenesis and that early embryonic cleavage is not required for A-V asymmetry preservation.

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Acknowledgements

The authors are grateful to all lab members of the Laboratory of Germ Cells, Faculty of Fisheries and Protections of Waters, University of South Bohemia in Ceske Budejovice for their help during the experiments. The authors thank the Ministry of Education, Youth and Sports of the Czech Republic project CENAKVA (LM2018099), Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370), Czech Science Foundation (20-23836S) and Grant Agency of the University of South Bohemia (036/2020/Z) for their financial support.

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Authors and Affiliations

  1. Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic

    Mujahid Ali Shah, Marek Rodina, Abdul Rasheed Baloch, Roman Franěk, Tomáš Tichopád & Martin Pšenička

  2. South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime, 798-4206, Japan

    Taiju Saito

  3. Laboratory of Gene Expression, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic

    Radek Šindelka & Viktoriia Iegorova

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  1. Mujahid Ali Shah
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  2. Taiju Saito
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  3. Radek Šindelka
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  4. Viktoriia Iegorova
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  5. Marek Rodina
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  6. Abdul Rasheed Baloch
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  9. Martin Pšenička
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Contributions

Conceptualization: MAS, MP; methodology: MAS, VI; formal analysis and investigation: RF, MR, TT; writing—original draft preparation: MAS; writing—review and editing: MAS, TS, RŠ, ARB, MP, VI; supervision: MP.

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Correspondence to Mujahid Ali Shah.

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Shah, M.A., Saito, T., Šindelka, R. et al. Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo. Fish Sci 87, 71–83 (2021). https://doi.org/10.1007/s12562-020-01481-7

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