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Naproxen/Layered Double Hydroxide Composites for Tissue-Engineering Applications: Physicochemical Characterization and Biological Evaluation

Published online by Cambridge University Press:  01 January 2024

Marcela P. Bernardo Open the ORCID record for Marcela P. Bernardo [Opens in a new window] ,
Bruna C.S. Rodrigues ,
Tamires D. de Oliveira ,
Adriana P.M. Guedes ,
Alzir A. Batista  and
Luiz H.C. Mattoso
Marcela P. Bernardo*
Affiliation:
National Nanotechnology Laboratory for Agribusiness, Embrapa Instrumentation, Brazilian Agricultural Research Corporation, São Carlos, São Paulo, Brazil
Bruna C.S. Rodrigues
Affiliation:
National Nanotechnology Laboratory for Agribusiness, Embrapa Instrumentation, Brazilian Agricultural Research Corporation, São Carlos, São Paulo, Brazil
Tamires D. de Oliveira
Affiliation:
Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, Brazil
Adriana P.M. Guedes
Affiliation:
Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, Brazil
Alzir A. Batista
Affiliation:
Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, Brazil
Luiz H.C. Mattoso
Affiliation:
National Nanotechnology Laboratory for Agribusiness, Embrapa Instrumentation, Brazilian Agricultural Research Corporation, São Carlos, São Paulo, Brazil
*
*E-mail address of corresponding author: marcelapiassib@gmail.com
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Abstract

Injured bone tissues can be healed with bone grafts, but this procedure may cause intense pain to the patient. A slow and localized delivery of nonsteroidal anti-inflammatory drugs (NSAIDs) could help to reduce the pain without affecting bone regeneration. The objective of the present study was to use [Mg-Al]-layered double hydroxide (LDH) as a matrix for controlled release of sodium naproxen (NAP). This system could be applied in biomaterial formulations (such as bone grafts) to achieve a local delivery of naproxen. [Mg-Al]-LDH successfully incorporated up to 80% (w/w) of naproxen by the structural reconstruction route, with the [Mg-Al]-LDH interlayer space increasing by 0.55 nm, corresponding to the drug molecule size. The evaluation of the naproxen release kinetics showed that 40% of the drug was delivered over 48 h in aqueous medium (pH 7.4 ± 0.1), indicating the potential of [Mg-Al]-LDH/NAP for local release of naproxen at adequate concentrations. Kinetic modeling showed that the naproxen release process was closely related to the Higuchi model, which considers the drug release as a diffusional process based on Fick’s law. The chemical stability of NAP after the release tests was verified by 1H NMR. The [Mg-Al]-LDH/NAP also exhibited low cytotoxicity toward fibroblast cells (L929 cell line), without modifications in their morphology and adhesion capacity. These results describe a suitable approach for preparing efficient systems for local delivery of nonsteroidal anti-inflammatory drugs for biomedical applications.

Keywords

BiomaterialsControlled releaseCytotoxicityHydrotalciteNSAIDStructural reconstruction
Type
Article
Information
Clays and Clay Minerals , Volume 68 , Issue 6 , December 2020 , pp. 623 - 631
Copyright
Copyright © Clay Minerals Society 2021

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