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Biochemical changes in microalga Porphyridium cruentum associated with silver nanoparticles biosynthesis

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Abstract

For the first time, the microalga Porphyridium cruentum was tested for its ability to produce silver nanoparticles. To characterize formed silver nanoparticles UV–vis Spectrometry, Scanning Electron Microscopy, Energy-dispersive analysis of X-rays and X-ray diffraction were used. It was shown that after biomass exposure to silver nitrate solution the extracellular formation of spherical-like nanoparticles took place. Functional groups responsible for metal binding were determined by Fourier-transform infrared spectroscopy. The complex of biochemical tests was used for biomass characterization and assessment of the changes of its main components (proteins, lipids, carbohydrates, and phycobilin) during nanoparticle formation. Obtained data indicate a significant decrease of proteins, carbohydrates, phycobiliproteins, and lipids content as well as antiradical activity of biomass. The obtained results show the necessity of determination of optimal conditions for obtaining Porphyridium cruentum biomass enriched with silver nanoparticles for its further application in the pharmaceuticals industry

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Data availability

The data sets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Institute of Microbiology and Biotechnology, 1, Academiei Str, 2028, Chisinau, Republic of Moldova

    Liliana Cepoi, Ludmila Rudi, Tatiana Chiriac, Vera Miscu & Valeriu Rudic

  2. Joint Institute for Nuclear Research, Joliot-Curie Str., 6, 1419890, Dubna, Russia

    Inga Zinicovscaia

  3. Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, 30 Reactorului Str. MG-6, Bucharest - Magurele, Romania

    Inga Zinicovscaia

Authors
  1. Liliana Cepoi
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  2. Ludmila Rudi
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  3. Inga Zinicovscaia
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  4. Tatiana Chiriac
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  5. Vera Miscu
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  6. Valeriu Rudic
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Contributions

All authors contributed to the study conception and design of the experiment and read and approved the final version of the manuscript.

Corresponding author

Correspondence to Inga Zinicovscaia.

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Communicated by Erko Stackebrandt.

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Cepoi, L., Rudi, L., Zinicovscaia, I. et al. Biochemical changes in microalga Porphyridium cruentum associated with silver nanoparticles biosynthesis. Arch Microbiol 203, 1547–1554 (2021). https://doi.org/10.1007/s00203-020-02143-z

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  • DOI: https://doi.org/10.1007/s00203-020-02143-z

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