Abstract
The golden chanterelle represents one of the commonly found, edible mushrooms that is highly valued in various cuisines. The present study focused on assessing the requirements of Cantharellus cibarius such as pH, temperature, as well as the carbon and nitrogen sources for mycelial growth. Optimization of the growth parameters was carried out by one-factor-at-a-time method. The optimal pH and temperature were determined to be 6.0 and 22.5 °C, respectively. Among the various carbon sources studied, sucrose at a concentration of 2% gave maximum mycelial growth and proved to be the most suitable one. Amongst the nitrogen sources studied, peptone, ammonium sulphate, and sodium nitrate, gave the maximum mycelial growth at an optimized concentration of 0.5%. In the presence of beef extract and yeast extract, a change in colony pigmentation from yellow to dark grey was observed. Finally, the carbon to nitrogen ratio of 2:0.5 proved to be optimal for mycelial growth. This study is the first report on the optimisation of in vitro growth requirements of C. cibarius.
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source on mycelial growth of C. cibarius. Data is given as mean of triplicates and error bars represent standard deviation. Data with the same data labels does not vary significantly (p < 0.05; Tukey’s HSD test)
source on mycelial growth of C. cibarius. Data is given as mean of triplicates and error bars represent standard deviation. Data with the same data labels does not vary significantly (p < 0.05; Tukey’s HSD test)
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The authors wish to thank Mr. Antti Kinnunen for administrative service and Mrs. Heli Valtonen for her technical assistance. We appreciate and are grateful for the financial support of Regional Council of Southern Savo, Finland.
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Deshaware, S., Marathe, S.J., Bedade, D. et al. Investigation on mycelial growth requirements of Cantharellus cibarius under laboratory conditions. Arch Microbiol 203, 1539–1545 (2021). https://doi.org/10.1007/s00203-020-02142-0
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DOI: https://doi.org/10.1007/s00203-020-02142-0