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Mesobacillus aurantius sp. nov., isolated from an orange-colored pond near a solar saltern

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Abstract

An endospore producing, strict aerobic, Gram-stain-positive, orange-colored colony forming bacterium designated as strain JC1013T was isolated from an orange pond near a solar saltern of Tamil Nadu, India. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain was affiliated to the family Bacillaceae of the phylum Firmicutes. Strain showed highest 16S rRNA gene sequence identity of 98.7% with Mesobacillus selenatarsenatis SF-1 T and below 98.3% with other members of the genus Mesobacillus. Strain JC1013T produced carotenoid pigments and indole compounds. Major cellular fatty acids of strain JC1013T were iso-C15:0, anteiso-C15:0, C16:0 3-OH, iso-C17:0ω10c and summed feature 4 (iso-C17:1 I/ anteisoB). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and four unidentified phospholipids. Strain JC1013T constituted m-diaminopimelic acid as diagnostic cell wall amino acids. MK-7 is the predominant menaquinone of strain JC1013T. The genome size of strain JC1013T was 4.6 Mbp and its G + C content was 42.7 mol%. For the affirmation of strain’s taxonomic status, a detailed phylogenomic study was done. Based on the phylogenetic analyses, low ANI (84.6%), AAI (88.5%) values, in-silico DDH (< 29%) value, morphological, physiological and chemo-taxonomical characteristics, strain JC1013T was clearly distinguished from the nearest phylogenetic neighbor, Mesobacillus selenatarsenatis SF-1T to conclude that it is a new species of the genus Mesobacillus. We propose the name as Mesobacillus aurantius with type strain JC1013T (= NBRC 114146T = KACC 21451 T).

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Abbreviations

NCBI:

National centre for biotechnology information

GCM:

The global catalogue of microorganisms

ANI:

Average nucleotide identity

AAI:

Average amino identity

dDDH:

Digital DNA–DNA hybridization

BLAST:

Basic local alignment search tool

MUSCLE:

Multiple sequence comparison by log-expectation

TLC:

Thin-layer chromatography

HPLC:

High-pressure liquid chromatography

NBRC:

Biological resource center

NITE:

KACC, Korean agricultural culture collection

CMC:

Carboxymethyl cellulose

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Acknowledgements

Anusha is grateful to the Department of Sciences and Technology, Government of India for the award of JRF/SRF Inspire fellowship. Ch.V.R. thanks the Department of Biotechnology, Government of India, for the award of Tata Innovative fellowship.Ch. S thank UGC, Government of India for Mid-Career award grant. Jagadeeshwari thanks TEQIP for research fellowship. All authors thank the BGI group (GCM 10K type strain sequencing project), China for genome sequencing of strain JC1013T. Financial support received from Council for Scientific and Industrial Research (CSIR), University Grants commission (UGC), All India Council for Technical Education (AICTE) New Delhi are acknowledged. Infrastructure facilities funded by DST-FIST, UGC-SAP (DRS) and TEQIP are acknowledged.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

    Anusha Rai, N. Smita, A. Shabbir, T. Keertana & Ch. V. Ramana

  2. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University H, Kukatpally, Hyderabad, 500 085, India

    U. Jagadeeshwari & Ch. Sasikala

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  1. Anusha Rai
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Correspondence to Ch. Sasikala or Ch. V. Ramana.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession number for 16S rRNA gene sequence of strain JC1013T is LS998022. The GenBank/EMBL/DDBJ accession for the whole genome shotgun sequence is JAAVUM000000000.

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Rai, A., Smita, N., Shabbir, A. et al. Mesobacillus aurantius sp. nov., isolated from an orange-colored pond near a solar saltern . Arch Microbiol 203, 1499–1507 (2021). https://doi.org/10.1007/s00203-020-02146-w

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