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Description of Cohnella zeiphila sp. nov., a bacterium isolated from maize callus cultures

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Abstract

A Gram-stain positive, aerobic, motile, rod-shaped bacterium designated as strain CBP-2801T was isolated as a contaminant from a culture containing maize callus in Peoria, Illinois, United States. The strain is unique relative to other Cohnella species due to its slow growth and reduced number of sole carbon sources. Phylogenetic analysis using 16S rRNA indicated that strain CBP-2801T is a Cohnella bacterium and showed the highest similarity to Cohnella xylanilytica (96.8%). Genome-based phylogeny and genomic comparisons based on average nucleotide identity confirmed the strain to be a novel species of Cohnella. Growth occurs at 15–45 °C (optimum 40 °C), pH 5–7 (optimum pH 6) and with 0–1% NaCl. The predominant fatty acids are anteiso-15:0 and 18:1 ω6c. Genome mining for secondary metabolites identified a putative biosynthetic cluster that encodes for a novel lasso peptide. In addition, this study contributes five new genome assemblies of type strains of Cohnella species, a genus with less than 30% of the type strains sequenced. The DNA G + C content is 58.7 mol %. Based on the phenotypic, phylogenetic and biochemical data strain CBP-2801T represents a novel species, for which the name Cohnella zeiphila sp. nov. is proposed. The type strain is CBP-2801T (= DSM 111598 = ATCC TSD-230).

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Acknowledgments

The authors thank David Lee, Heather Walker and Mark Doehring of the National Center for Agricultural Utilization Research (U.S. Department of Agriculture) for helpful technical assistance. We appreciate Alejandro Rooney for encouraging this research. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer.

Funding

Funding for this work was provided under USDA in-house projects #5010-22410-017-00-D and #5010-22410-019-00-D.

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Authors and Affiliations

  1. Crop Bioprotection Research, USDA Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA

    Eric T. Johnson, Patrick F. Dowd & Christopher A. Dunlap

  2. Renewable Product Technology Research, USDA Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA

    Christopher D. Skory

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  1. Eric T. Johnson
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  2. Patrick F. Dowd
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  3. Christopher D. Skory
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  4. Christopher A. Dunlap
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EJ, PD and CD equally contributed to this work. CS contributed expertise in microscopy.

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Correspondence to Eric T. Johnson.

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Johnson, E.T., Dowd, P.F., Skory, C.D. et al. Description of Cohnella zeiphila sp. nov., a bacterium isolated from maize callus cultures. Antonie van Leeuwenhoek 114, 37–44 (2021). https://doi.org/10.1007/s10482-020-01495-2

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