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Characterization and Biotechnological Functional Activities of Exopolysaccharides Produced by Lysinibacillus fusiformis KMNTT-10

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Abstract

In this study, the production and physicochemical characterization of exopolysaccharide (EPS) produced by Lysinibacillus fusiformis KMNTT-10 were investigated. Also, in vitro assays were conducted to investigate the biotechnological functional activities of EPS, including flocculation, emulsification, and antioxidant activity. Results showed that the maximum EPS produced by strain KMNTT-10 was 4.19 g/l after 120 h. The monosaccharides present in EPS were xylose, rhamnose, arabinose, galactose, and glucose in varying combinations. Characterization of EPS by FTIR and XRD analysis revealed the presence of hydroxyl, amino, and carboxyl functional groups and further, the EPS is an amorphous nature. SEM envisaged a highly porous web-like structure with an irregular surface in the EPS morphology. Further, the EPS had good flocculation activity with a maximum of 89.66% and also had potent emulsifying activity towards hydrocarbons with 1% EPS concentration. The EPS showed good antioxidant activity by potent scavenging actions on DPPH, ABTS, and nitric oxide free radicals. Overall results demonstrated that EPS of KMNTT-10 could be used in various industrial applications due to its excellent flocculation, emulsification potential, and good antioxidant activity.

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Acknowledgements

We would like to thank major research of “Mechanism underlying elemental cycling on the earth by microorganisms in hydrosphere” (Grant No. 91851206) launched by NSFC and the National Nature Science Foundation of China (NSFC: Grant No. 41877345). The Postdoctoral research plan at Central South University for K. Mathivanan is gratefully acknowledged. We wish to thank the Hunan International Scientific and Technological Cooperation Base of Environmental Microbiome and Application [2018WK4019] for funding the research.

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, People’s Republic of China

    Krishnamurthy Mathivanan, Delong Meng & Huaqun Yin

  2. Environmental Nanotechnology Division, Sri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tamil Nadu, 627 412, India

    Jayaraman Uthaya Chandirika

  3. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, People’s Republic of China

    Annadurai Vinothkanna

  4. Guangdong Province Key Laboratory of Microbial Signals and Disease Control and Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    R. K. Govindarajan

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  1. Krishnamurthy Mathivanan
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  4. R. K. Govindarajan
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Contributions

MK: conceptualiation, Investigation, Methodology, Data curation, Writing-original draft. JUC: formal analysis. AV: data curation. RKG: formal analysis. DM: validation. HY: conceptualiation, funding acquisition, resources, and supervision.

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Correspondence to Krishnamurthy Mathivanan or Huaqun Yin.

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Mathivanan, K., Chandirika, J.U., Vinothkanna, A. et al. Characterization and Biotechnological Functional Activities of Exopolysaccharides Produced by Lysinibacillus fusiformis KMNTT-10. J Polym Environ 29, 1742–1751 (2021). https://doi.org/10.1007/s10924-020-01986-3

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