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In-Silico and In-Vitro Evaluation of Antibacterial, Cytotoxic, and Apoptotic Activity and Structure of Modified CM11 Peptide

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Abstract

Drug-resistant infectious diseases have increased in recent years. Accordingly, plenty of researches are exploring novel approaches to overcome this problem. In this era, antimicrobial peptides have been identified as potential antibacterial agents. The Modified CM11 (mCM11) was designed with the C-terminal amidation and substitution of lysine with arginine. The designed peptide was synthesized by the solid-phase method and Rink amide p-methyl-benzhydryl amine resin. The synthesized peptide was evaluated using Mass Spectrometry (MS), High-Performance Liquid Chromatography (HPLC), and Circular Dichroism (CD). Finally, the antibacterial, cytotoxic, and apoptotic effect of the mCM11 peptide was investigated. The new peptide indicated a beta-sheet structure with a molecular weight of 1527.50 D and purity of 96%. The peptide exerted a potent antimicrobial activity against gram-negative and positive bacteria. The minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) ranged from 16 to 64 µg/ml, and 16 to 128 µg/ml, respectively. The IC50 of mCM11 was 16 µg/ml and its cytotoxicity in SH-SY5Y cell line revealed a dose-dependent manner. Also, apoptosis analysis of eukaryotic cells revealed a decline in late apoptosis and necrosis in comparison with untreated cells. The mCM11 indicated a considerable antibacterial effect against a wide range of pathogenic bacterial strains. Further, it did not represent any late apoptotic and necrosis impact on the eukaryotic cell line. All of these findings may confirm the potential role of this new peptide as an effective therapeutic agent.

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Acknowledgments

This study was conducted on the scientific and spiritual support of the Islamic Azad University of Qom. The authors would also like to thank Mr. and Mrs. Mohammad Eshtiaghi, Mansoureh Algharnia, Samaneh Banaeian Kalaat, Zahra Karimi, Fahimeh Afzali, and all professors and students who helped with the projects.

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Authors and Affiliations

  1. Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

    Sajjad Eshtiaghi & Razieh Nazari

  2. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mahdi Fasihi-Ramandi

Authors
  1. Sajjad Eshtiaghi
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  2. Razieh Nazari
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  3. Mahdi Fasihi-Ramandi
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Correspondence to Razieh Nazari.

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"Qom branch of Islamic Azad University" did not play any decision-making role in the study analysis or writing of the manuscript. All authors declare no Potential Conflicts of Interest.

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As the present study involved no experimental with animals or humans, hence there was no need for approval by the Ethics Committee of Islamic Azad University, Qom branch.

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Eshtiaghi, S., Nazari, R. & Fasihi-Ramandi, M. In-Silico and In-Vitro Evaluation of Antibacterial, Cytotoxic, and Apoptotic Activity and Structure of Modified CM11 Peptide. Int J Pept Res Ther 27, 1069–1078 (2021). https://doi.org/10.1007/s10989-020-10151-2

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  • DOI: https://doi.org/10.1007/s10989-020-10151-2

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