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Wound Healing Activity of Phage-Sisplayed TGF-β1 Model Peptide in Streptozotocin-Induced Diabetic Rats

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International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

Abstract

Objective

To evaluate the effect of phage-displayed TGF-β1 model peptide on cutaneous wound healing in streptozotocin-induced diabetic rats.

Methods

Full-thickness excisional wounds were made on the dorsums of 50 rats which were then randomly divided into five groups: negative control group (normal saline (NS)), two TGF-β1 control groups which were respectively treated with low-dose TGF-β1 ( 5 ng/ml) and high-dose TGF-β1 (50 ng/ml), and two model-peptide-treated groups which were respectively treated with low-dose model peptide ( 5 ng/ml,) and high-dose model peptide (50 ng/ml). At day 14 post-injury, rats were euthanised and wounds were assessed by gross, histopathology, immunohistochemistry, immunofluorescence and quantificational real-time polymerase chain reaction.

Results

A significant increase in rate of wound closure was observed in model peptide groups in comparison to negative control group. The results of histopathological staining revealed that re-epithelization and collagen deposition in model-peptide-treated groups were significantly higher than those in negative control group. The results of immunohistochemistry and immunofluorescence tests showed that Ki67-positive, VEGFA-positive, CD31-positive, α-SMA-positive, CD206-positive cells in model peptide and TGF-β1 control groups were more than those in negative control group. Furthermore, comparing with the mRNA expression profile in negative control groups, mRNA expression profile in model peptide group showed a decrease in proinflammatory cytokine and an increase in anti-inflammatory cytokine and collagen.

Conclusions

Phage-displayed TGF-β1 peptide facilitates wound healing through accelerating re-epithelialization, enhancing collagen deposition, promoting neo-vascularization, and inhibiting inflammatory response. Model peptide possesses the potential to be a promising treatment strategy for enhancing diabetic wound repair.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (30670571, 81201467), and the Scientific Research Fund for Youth of Chinese Academy of Medical Sciences and Peking Union Medical College (2017310007). Otherwise, all the authors declare that they have no financial or personal interference with other people or organizations that could inappropriately influence their work.

Funding

This study was supported by the National Natural Science Foundation of China (30670571, 81201467), and the Scientific Research Fund for Youth of Chinese Academy of Medical Sciences and Peking Union Medical College (2017310007).

Author information

Authors and Affiliations

  1. 16Th Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Hong Du, Guodong Song, Dong Zhang, Panxi Yu, Chenzhi Lai, Xiaoshuang Guo, Xianlei Zong & Xiaolei Jin

  2. Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, 247 Beiyuan Road, Tianqiao District, Jinan, 250033, Shandong, China

    Duyin Jiang

  3. Department of Scientific Research, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Chunyan Cao

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  1. Hong Du
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  2. Duyin Jiang
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  5. Dong Zhang
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  8. Xiaoshuang Guo
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  9. Xianlei Zong
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  10. Xiaolei Jin
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Correspondence to Xianlei Zong or Xiaolei Jin.

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The authors declare that there are no conflicts of interest.

Ethics Approval

The current research was approved by the Medical Ethical Committee at Plastic Surgery Hospital of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Research Involving Human and Animal Participants

50 female Sprague–Dawley (SD) rats were obtained from Beijing Medical Laboratory Animal Center (SYXK 2020–0018). Fibroblasts were obtained from a healthy patient underwent abdominoplasty.

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Informed consent was given by the patient who donated skin for cell culture.

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Du, H., Jiang, D., Song, G. et al. Wound Healing Activity of Phage-Sisplayed TGF-β1 Model Peptide in Streptozotocin-Induced Diabetic Rats. Int J Pept Res Ther 27, 1079–1094 (2021). https://doi.org/10.1007/s10989-020-10152-1

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