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Vibrio marinisediminis sp. nov., Isolated from Marine Sediment

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Abstract

A novel Gram-stain-negative, facultative anaerobic, motile bacterium, designated as 404T, was isolated from a marine sediment sample in the Bohai Gulf, China. Growth was observed at 10–35 °C (optimum, 20–25 °C) and in the presence of 1.0–6.0% (w/v) NaCl (optimum, 1.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 404T belonged to the genus Vibrio, showing the highest sequence similarity to Vibrio renipiscarius KCTC 42287T (97.6%). The draft genome is 4.5 Mb in length, containing 4278 protein-coding genes, 60 tRNA genes and 9 rRNA genes, and DNA G+C content is 44.1 mol%. Strain 404T contains phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, and phospholipid as the main polar lipids, and the predominant quinone is ubiquinone Q-8. The major cellular fatty acids (>8.0%) are C16 : 1ω6c and/or C16 : 1ω7c, C16 : 0, C18 : 1ω6c and/or C18 : 1ω7c. Strain 404T shows some typical characteristics among the members of genus Vibrio, while it can be clearly distinguished from the closely related type strains through genome analysis (average nucleotide identity and digital DNA–DNA hybridization values), fatty acid composition and a series of physiological and biochemical characteristics. On the basis of the polyphasic analysis, strain 404T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio marinisediminis sp. nov., is proposed. The type strain is 404T (= MCCC 1H00367T = KCTC 62958T).

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Acknowledgements

We sincerely thank Prof. Aharon Oren in Hebrew University of Jerusalem for advice on nomenclature. This work was supported by the National Natural Science Foundation of China (81702054, 81501718) and Natural Science Foundation of Shandong Province (ZR2017BC011).

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  1. Huilin Zhao and Jiangfan Shan contributed equally to the paper.

Authors and Affiliations

  1. Department of Pathogenic Biology, School of Basic Medical Sciences, Binzhou Medical University, Yantai, China

    Huilin Zhao, Jiangfan Shan, Yue Tian, Boqing Li & Xiaofei Ji

  2. College of Marine Science, Shandong University at Weihai, Weihai, China

    Taojie Wang & Zongjun Du

  3. School of Pharmacy, Binzhou Medical University, Yantai, China

    Yingjian Shen

  4. State Key Laboratory of Microbial Technology, Shandong University, Jinan, China

    Zongjun Du

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  1. Huilin Zhao
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Correspondence to Boqing Li or Xiaofei Ji.

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The GenBank accession numbers of Vibrio marinisediminis 404T are MK347066 (16S rRNA gene) and JACFYF000000000 (draft genome), respectively.

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Zhao, H., Shan, J., Wang, T. et al. Vibrio marinisediminis sp. nov., Isolated from Marine Sediment. Curr Microbiol 78, 810–815 (2021). https://doi.org/10.1007/s00284-020-02290-y

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