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Promotion of the Asymmetric Reduction of Prochiral Ketone with Recombinant E. coli Through Strengthening Intracellular NADPH Supply by Modifying EMP and Introducing NAD Kinase

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Abstract

The intracellular NADPH insufficient supply is the main bottleneck to the synthesis of chiral alcohols by asymmetric reduction with whole-cell catalysis. Herein, we provide a novel strategy to strengthen intracellular NADPH supply through introducing an NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase (gapB from Bacillus subtilis 168) into the Embden-Meyerhof pathway and a NAD kinase (yfjB from E. coli MG1655) to further enhance the NADP(H) pool. A recombinant E. coli (E. coli BL21 (DE3)/pETDuet-1-gapB-yueD&pET28a-yfjB) was constructed to co-express gapB and yfjB with a carbonyl reductase gene yueD together. The result showed that the intracellular NADPH amount increased by 134.4% with the strategy. To the model reaction (asymmetric reduction of acetophenone to S-phenyl ethanol), the yield was 3.7-fold with this strategy compared to the control. This provides a technological route for strengthening the intracellular NADPH supply in E. coli for biocatalysis and biosynthesis.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Grant No. 51608400) and the Educational Commission of Hubei Province of China (Grant No. D20121108).

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Author notes

  1. Hui-Jun Du and Wei Luo have contributed equally to this work and should be considered as co-first authors.

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Postal box 154#, Wuhan, 430081, China

    Hui-Jun Du, Wei Luo, Bright Appiah, Zhi-Cheng Zou & Zhong-Hua Yang

  2. College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

    Rong Zeng

  3. School of Agriculture, Hubei Three Gorges Polytechnic, Yi Chang, 443000, China

    Li Luo

  4. Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, Wuhan University of Science and Technology, Wuhan, 430081, China

    Zhong-Hua Yang

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  1. Hui-Jun Du
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  4. Zhi-Cheng Zou
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Correspondence to Zhong-Hua Yang, Rong Zeng or Li Luo.

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Du, HJ., Luo, W., Appiah, B. et al. Promotion of the Asymmetric Reduction of Prochiral Ketone with Recombinant E. coli Through Strengthening Intracellular NADPH Supply by Modifying EMP and Introducing NAD Kinase. Catal Lett 151, 2527–2536 (2021). https://doi.org/10.1007/s10562-020-03490-8

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  • DOI: https://doi.org/10.1007/s10562-020-03490-8

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