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Box–Behnken design optimisation of a green novel nanobio-based reagent for rapid visualisation of latent fingerprints on wet, non-porous substrates

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Abstract

Objective

Optimisation of the green novel nanobio-based reagent (NBR) for rapid visualisation of groomed fingerprints on wet non-porous substrates using response surface methodology and assessment of its stability and sensitivity were attempted for forensic applications.

Results

Scanning electron microscopy images demonstrated successful attachments of NBR onto the constituents of fingerprints on the substrates. The highest average quality of visualised fingerprints was attained at the optimum condition (100 mg of CRL; 75 mg of acid-functionalised multi-walled carbon nanotubes; 5 h of immobilisation). The NBR produced comparable average quality of fingerprints with the commercially available small particle reagent, even after 4 weeks of storage (without any preservatives) in both chilled and sultry conditions. The NBR was sensitive enough to visualise the increasingly weaker fingerprints, particularly on glass slides.

Conclusion

The optimised novel NBR could be the relatively greener option for visualising latent fingerprints on wet, non-porous substrates for forensic applications.

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Acknowledgements

The authors are thankful to the Universiti Teknologi Malaysia and Ministry of Higher Education (Malaysia) for providing the Research University Grant (Q.J130000.2526.12H51) and Fundamental Research Grant Scheme (R.J130000.7854.4F990) for conducting a research project on visualisation of latent fingerprints on immersed non-porous substrates using lipase reinforced nanoconjugates, respectively. The authors are also thankful to all of the fingerprint donors as well as Syafiza Shari’at, Nadirah Suliman, Ain Naqwa and Anis Syahirah for the kind assistance throughout this research.

Funding

Universiti Teknologi Malaysia and Ministry of Higher Education (Malaysia) for providing the Research University Grant (Q.J130000.2526.12H51) and Fundamental Research Grant Scheme (R.J130000.7854.4F990), respectively.

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Correspondence to Naji Arafat Mahat or Roswanira Abdul Wahab.

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Conflict of interest

The authors declared no conflict of/competing interest.

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The ethical approval was Granted by the Research Ethics Committee (Human) of Universiti Sains Malaysia (USM/JEPeM/19010069).

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Azman, A.R., Mahat, N.A., Wahab, R.A. et al. Box–Behnken design optimisation of a green novel nanobio-based reagent for rapid visualisation of latent fingerprints on wet, non-porous substrates. Biotechnol Lett 43, 881–898 (2021). https://doi.org/10.1007/s10529-020-03052-3

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  • DOI: https://doi.org/10.1007/s10529-020-03052-3

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