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Enhancement Propagation of Protocorms in Orchid (Cymbidium tracyanum L. Castle) by Cold Atmospheric Pressure Air Plasma Jet

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Abstract

Propagation of orchid (Cymbidium tracyanum L. Castle) treated by using an air cold atmospheric pressure plasma jet at room temperature was investigated. The peak-to-peak voltage discharge and flow rate of the air atmospheric pressure plasma jet were set at 10.5 kV and 10 L/min, respectively. The constituent particles of the air cold atmospheric pressure plasma jet were revealed by optical emission spectroscopy. At the end of the jet nozzle, the constituent particles were observed, such as hydroxyl radical ˙OH, N2 first negative system (N2 1 −), N2s positive system (N2 2 +), and atomic oxygen. The average size, average number of buds, fresh and dry weights simultaneously increased by cold air plasma treatment compared with the control ones. The highest positive effects of treated protocorms on average size, average number of buds, fresh and dry weight were significantly improved by 238.2, 475.9, 85.6, and 152.5%, respectively. The transmission electron microscope showed the cytoplasmic cell wall in orchid protocorm disturbed by air cold plasma. Thus, the effects of constituent particles of air plasma might attack in the cell wall of protocorm leading to cell loosening, which improved elongation and released bud dormancy. These results indicated that the propagation of orchid protocorms depended on the effects of air cold plasma treatment with different plasma time durations.

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Acknowledgements

We thank the financially supported by the RMUTT Research Foundation Scholarship in 2019, and the RMUTT annual government statement of expenditure in 2020.

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Authors and Affiliations

  1. Department of Materials and Metallurgical Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand

    Sorapong Pavasupree

  2. Expert Center of Innovative Agriculture (InnoAg), Thailand Institute of Scientific and Technological Research (TISTR), Technopolis, Khlong Ha, Khlong Luang, Pathumthani, Thailand

    Nattapong Chanchula

  3. Division of Physics, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand

    Atipong Bootchanont, Chakkaphan Wattanawikkam & Porramain Porjai

  4. Electrical Engineering Department, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand

    Pinit Jitjing

  5. Plasma and Beam Physics Research Facility, Chiang Mai University, 239 Huay Kaew Rd., Chiang Mai, 50200, Thailand

    Dheerawan Boonyawan

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  1. Sorapong Pavasupree
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Pavasupree, S., Chanchula, N., Bootchanont, A. et al. Enhancement Propagation of Protocorms in Orchid (Cymbidium tracyanum L. Castle) by Cold Atmospheric Pressure Air Plasma Jet. Plasma Chem Plasma Process 41, 573–589 (2021). https://doi.org/10.1007/s11090-020-10148-1

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