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18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis

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Abstract

GP64 is the key membrane fusion protein of Group I baculovirus, and while the Bombyx mori nucleopolyhedrovirus (BmNPV) GP64 contains a longer n-region (18 amino acid) of the signal peptide than does the Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the function of the n-region has not been determined. In this study, we first showed that n-region is required for membrane protein localization in BmN cells, then the transcriptome sequencing was conducted on proteins guided by different signal peptide regions, and the results were analyzed and validated by quantitative PCR and luciferase assays. The results indicated that 1049 differentially expressed genes (DEGs) were identified among the different region of signal peptides and the control. With the n-region, the protein export pathway was upregulated significantly, the Wnt-1 signaling pathway was downregulated, and BiP was significantly activated by the GP64 full-length signal peptide. Furthermore, RNA interference on BiP efficiently increased luciferase secretion. These results indicate that the GP64 n-region plays a key role in protein expression and regulation.

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Acknowledgements

This work was supported by the National Science Foundation of China (Grant Nos. 31670152, 81802083).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, People’s Republic of China

    Na Liu, Jinshan Huang, Lin Liu, Frank Boadi, Xingjia Shen & Bifang Hao

  2. Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, 215031, Jiangsu, People’s Republic of China

    Yanhui Song

  3. Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Zhongjian Guo

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  1. Na Liu
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Contributions

Conceived and designed the study: JS H, YH S, ZJ G, and XJ S. Collected and analyzed the data: NL, and LL. Wrote and edited the manuscript: NL, FB, and BF H.

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Correspondence to Bifang Hao.

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Liu, N., Huang, J., Liu, L. et al. 18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis. Curr Microbiol 78, 490–501 (2021). https://doi.org/10.1007/s00284-020-02309-4

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