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Halomonas humidisoli Sp. Nov., Isolated From Saline–Alkaline Soil

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Abstract

A Gram staining-negative, halophilic, aerobic, non-motile bacteria, designated strain WN018T, were isolated from the natural saline–alkali wetland soil of Binhai new district, Tianjin, China (38°46′N, 117°13′E). Cells of strain WN018T were short rod-shaped, 0.3–0.4 µm wide and 0.5–1.9 µm long, and growth occurred optimally at 30–33 °C, pH 7.5–8.0, and in the presence of 4–8% (w/v) NaCl. Based on 16S rRNA gene sequence analysis, the isolates could be affiliated to the genus Halomonas, exhibiting highest sequence similarity of 97.50% to Halomonas venusta DSM 4743T. The DNA G+C content of the strain was 63.8%. The distinct phylogenetic position and phenotypic traits distinguished the novel isolate from its nearest neighbors. The major respiratory quinone of strain WN018T was Q-9 (91.0%) and Q-8 (9.0%), and the dominant fatty acids were C16:0, C14:0, C10:0, C12:0 3-OH. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), three phospholipids (PL), aminolipid (AL), and two unidentified lipids (L). The average nucleotide identity (ANI) based on whole-genome sequences of strain WN018T and Halomonas hydrothermalis DSM 15725T was 93.02%, and the dDDH value between these two strains was determined to be 49.7%. Therefore, we propose a novel species in the genus Halomonas to accommodate the novel isolate: Halomonas humidisoli sp. nov. (type strain WN018T = ACCC 19975T = KCTC 52854T).

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Acknowledgements

We would like to thank Prof. Aharon Oren for very valuable help in naming the organism.

Funding

This work was supported by the National Natural Science Foundation of China (NSFC No. 31670113) and National Key Research and Development Program of China (2017YFD0201401).

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Authors and Affiliations

  1. College of Life Science, Yangtze University, Jingzhou, Hubei, 434025, People’s Republic of China

    Hanxu Liu, Liu Tang & Lian Ma

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affair, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Hanxu Liu, Liu Tang, Juanjuan Zhao, Shaohua Miao, Qi Gong & Guishan Zhang

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  1. Hanxu Liu
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  2. Liu Tang
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  3. Juanjuan Zhao
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  4. Shaohua Miao
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  6. Lian Ma
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  7. Guishan Zhang
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Contributions

HL contributed to performing the experiments and writing the initial draft. GZ and LM contributed to the guidance of experimental operations. LT and SM contributed to the morphological analyses. JZ and QG performed genome analysis. GZ and LM contributed to reagents, instrumentation and the financial support for this work. All authors approved the manuscript.

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Correspondence to Lian Ma or Guishan Zhang.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene of Halomonas humidisoli sp. nov. strain WN018T is MF782431. The whole genome been deposited at DDBJ/EMBL/GenBank under the accession NSKD00000000 for strain WN018T Transmission electron micrographs (TEM) of cells of strain WN018T, thin–layer chromatograms of the polar lipids extracted from strain WN018T and closely related species, additional phylogenetic trees, and the table containing the average nucleotide identity (ANI), and dDDH values to closely related genomes are available as Supplementary Materials.

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Liu, H., Tang, L., Zhao, J. et al. Halomonas humidisoli Sp. Nov., Isolated From Saline–Alkaline Soil. Curr Microbiol 78, 803–809 (2021). https://doi.org/10.1007/s00284-020-02291-x

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  • DOI: https://doi.org/10.1007/s00284-020-02291-x

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